Coffee Science_v.19, 2024
URI permanente para esta coleção${dspace.url}/handle/123456789/14639
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Item The application of arbuscular mycorrhizal fungi and mycorrhizae helper bacteria combined with compost to reduce the Pratylenchus coffeae population in an Arabica coffee plantation(Editora UFLA, 2024-10-31) Hindersah, Reginawanti; Asyiah, Iis NurArbuscular mycorrhizal fungi (AMF) and mycorrhiza helper bacteria (MHB) are potential biological agents that control Pratylenchus coffeae, a major endoparasitic nematode in coffee plantations. An experiment was conducted to determine the role of AMF and MHB inoculants combined with compost in reducing plant damage and the number of nematodes in the soil and roots, as well as increasing the AMF infection rate in juvenile (JP) and mature (MP) Arabica coffee plants. The field experiment with a randomized block design was conducted with both juvenile (JP) and mature plants, consisting of 8 combination treatments and one control with three replications. The JP and MP plants received 1.5-50 g and 25-100 g AMF inoculant/plant and compost at doses of 5 or 7.5 and 10 or 15 kg/plant, respectively, while the MHB liquid inoculant concentrations were 10 mL and 100 mL, respectively. The control plants were not treated with AMF, MHB, or compost. The AMF and MHB inoculants combined with compost reduced leaf and root damage, but increased the degree of AMF infection in the roots of JP and MP. The nematode populations in roots and shoots of treated JP were reduced by 48.7-74.5% and 56.0 80.5%, respectively; in mature plants, the reductions ranged from 40.5 to 74.4% in roots and 44.0 to 75.4% in the soil. These results suggest that AMF, MHB, and compost effectively reduced P. coffeae infection in Arabica coffee plants under field conditions.Item Characterization in populations of Coffea arabica L. for resistance to CBD using molecular markers(Editora UFLA, 2024-09-24) López-Monsalve, Luisa F.; Quiroga-Cardona, Julio; López, Natalia Arango; Ramírez-Cardona, Carlos A.; Flórez-Ramos, Claudia P.Coffee berry disease (CBD) is caused by the fungus Colletotrichum kahawae Waller and Bridge. This disease is restricted to the African continent, where it can cause production losses of more than 80% when susceptible varieties are used or when the indicated chemical control is not carried out. For this reason, since 1970, Cenicafé has developed lines resistant to this disease in the absence of the pathogen, a process that has been favored by the discovery and validation of microsatellite markers associated with the Ck-1 gene for resistance to CBD. In this research, 12 populations of Coffea arabica were characterized for their resistance to CBD using the molecular markers Sat235, Sat207 and FR34-6CTG. The molecular markers allowed us to identify that the same allelic form of resistance to CBD is present in lines derived from Timor Hybrid CIFC 1343 (HdT CIFC 1343). Furthermore, the allelic form of resistance associated with the three molecular markers was identified in one line derived from Coffea canephora. In lines derived from Caturra x HdT CIFC 1343 it was evident that, when the plants present the allelic forms of resistance identified by the molecular markers, high percentages of hypocotyls resistant to different isolates of C. kahawae are observed in the progeny.