Coffee Science_v.19, 2024

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Analysis of shape features by applying gain ratio and machine learning for coffee bean classification
(Universidade Federal de Lavras, 2024-06-28) Septiarini, Anindita; Hamdani, Hamdani; Sela, Enny Itje; Hidayat, Nurul; Afuan, Lasmedi
Coffee is one of the daily consumed beverages in many countries. It is yielded from coffee beans, which have proceeded through several processes. Several common coffee beans have been produced in Indonesia, such as Arabica, Robusta, Liberica, and Excelsa. Nevertheless, many coffee fanatics are unable to distinguish the various coffee bean types visually based on those shapes. Accordingly, it is necessary to classify the types of coffee beans. The work applied training and testing steps. Both involved ROI detection, pre-processing, segmentation, feature extraction, selection, and classification. Image processing was used in ROI detection, pre-processing, and segmentation to simplify the procedure and separate the coffee bean from the background. The feature extraction produced 14 shape features to distinguish the coffee bean’s class, but the proposed method’s performance has yet to reach the optimal result. The gain ratio was used to reduce the features; hence, only 4 features were selected, including aspect ratio, eccentricity, equivalent diameter, and area. These features were utilized as input data for classification using Naive Bayes, Artificial Neural Network (ANN), Support Vector Machine (SVM), C4.5, and decision tree. The proposed method used 4 features and a decision tree classifier. The local dataset has 400 coffee bean photos in four classes of 100 images each. The photos were divided for training and testing using k-fold 10 cross-validation. The accuracy evaluation parameter reached 0.995.
Can season and intercropping Conilon coffee favor predatory ant species?
(Universidade Federal de Lavras, 2024-06-18) Martins, David dos Santos; Fornazier, Maurício José; Abonizio-Santos, Marlina Ribeiro; Guarçoni, Rogério Carvalho; Teixeira, Alex Fabian Rabelo; Magalhães, Fabrício Severo; Souza-Campana, Débora Rodrigues; Maria Santina de Castro, Maria Santina de Castro
Conilon coffee (Coffea canephora Pierre ex A. Froehner) is an important crop in Brazil, and this culture is widely grown in Espírito Santo State. Intercropping this species has been adopted for better use, profitability, and sustainability of the cultivated area. Intercropping provides higher shading and may favor edaphic fauna that plays an important ecological function in the soil. Ants are part of the edaphic meso - and macrofauna, considered ecosystem engineers and actively participate in pest predation. The goal of the study was to evaluate predatory ant richness in Conilon coffee in monoculture and intercropped with teak or Australian cedar. Furthermore, the influence of these crops on the richness of predator ant communities in the dry (winter) and rainy (summer) seasons was analyzed. The study was conducted in the municipality of Sooretama, northern Espírito Santo State (Brazil) using pitfall soil traps. Ant communities were analyzed with their distribution in trophic guilds. The richness of predatory ants (predatory and generalist omnivore predatory ant species) was studied in Conilon coffee in monoculture and intercropped with teak (Tectona grandis L.f.) and with Australian cedar (Toona ciliata M. Roemer), in the dry (winter) and wet (summer) seasons. A total of 49 ant species were collected, 29 predatory and 15 generalist omnivore predatory ant species were identified, and 16 ant species were recorded for the first time in Espírito Santo State. Conilon coffee cropping systems did not affect the richness of predatory or generalist omnivore predatory ant species, however, summer favored the occurrence of total predatory ants (generalist omnivore + predators); intercropping Conilon coffee did not favor predatory ants. The presence of predatory species is important for natural pest control, consequently improving crop sustainability. However, new research must be done to evaluate other Conilon coffee intercropping for a longer period.
Changes in the Chemical Compound and Sensory Profiles of Liberica Fermentation (Coffea liberica) with Cellulolytic Bacteria Alcaligenes sp and Exiguobacterium indicum
(Editora UFLA, 2024-11-14) Tarigan, Indra Lasmana; Adriliana, Zairaini; Putri, Alya Adiningtyas; Febrianto, Noor Ariefandie; Latief, Madyawati; Sutrisno
Jambi Province, particularly in Tanjung Jabung Barat Regency, Indonesia, is a crucial producer of Liberica coffee known as Liberika Tungkal Komposit (Libtukom). This variety of Liberica coffee (Coffea liberica) thrives in lowland regions, particularly peatlands. It grows best in slightly acidic soil with a pH between 6.0 and 6.5, which helps the plant absorb nutrients and resist pests more effectively. A significant post-harvest method to improve the quality and yield of Liberica coffee involves fermentation using cellulolytic bacteria, Alcaligenes sp. And Exiguobacterium indicum, found in civet digestive tracts. Fermentation enhances the coffe’s flavor, aroma, and quality, mimicking that of civet coffee. The primary objective of this study was to refine the sensory characteristics of Liberica coffee, improve its proximate composition, preserve its bioactive compounds, and boost its antioxidant properties. The fermentation was conducted over 24 hours and 48 hours periods, with roasting temperatures set at light (170 ºC), medium (190 ºC), and dark (210 ºC). The process began with the preparation of bacterial inoculum, followed by fermentation, roasting, and grinding. Sensory evaluation, proximate analysis, and chemical profiling (via FTIR, LC-MS, antioxidant activity, and total phenolic content) were then performed. The findings revealed that fermented Liberica coffee exhibited improved sensory attributes, achieving specialty-grade status at medium roasting temperatures. There was a decrease in moisture and ash content, an increase in fat content, and a reduction in carbohydrate and protein levels. The FTIR analysis showed the preservation of existing compounds, while LC-MS identified bioactive components based on their retention times and molecular weights. Additionally, the fermented coffee demonstrated increased antioxidant activity and total phenolic content compared to non-fermented samples. This study highlights the potential of fermentation technology to enhance the quality and health benefits of Liberica coffee significantly.
Characterization in populations of Coffea arabica L. for resistance to CBD using molecular markers
(Editora UFLA, 2024-09-24) López-Monsalve, Luisa F.; Quiroga-Cardona, Julio; López, Natalia Arango; Ramírez-Cardona, Carlos A.; Flórez-Ramos, Claudia P.
Coffee berry disease (CBD) is caused by the fungus Colletotrichum kahawae Waller and Bridge. This disease is restricted to the African continent, where it can cause production losses of more than 80% when susceptible varieties are used or when the indicated chemical control is not carried out. For this reason, since 1970, Cenicafé has developed lines resistant to this disease in the absence of the pathogen, a process that has been favored by the discovery and validation of microsatellite markers associated with the Ck-1 gene for resistance to CBD. In this research, 12 populations of Coffea arabica were characterized for their resistance to CBD using the molecular markers Sat235, Sat207 and FR34-6CTG. The molecular markers allowed us to identify that the same allelic form of resistance to CBD is present in lines derived from Timor Hybrid CIFC 1343 (HdT CIFC 1343). Furthermore, the allelic form of resistance associated with the three molecular markers was identified in one line derived from Coffea canephora. In lines derived from Caturra x HdT CIFC 1343 it was evident that, when the plants present the allelic forms of resistance identified by the molecular markers, high percentages of hypocotyls resistant to different isolates of C. kahawae are observed in the progeny.
Chemical and microbiological characteristics of kombucha made from robusta cascara and green tea
(Universidade Federal de Lavras, 2024-06-17) Parwiyanti; Nurrocmah, Siti Iliyo; Malahayati, Nura
Dried coffee peel or cascara as coffee waste is a potential ingredient that still contains phytochemical substances such as polyphenol and clorogenic acid. Cascara can be used in making kombucha. Kombucha is a functional drink made of fermenting tea and sugar with using symbiotic culture of bacteria and yeast (SCOBY) as starter. The addition of cascara and combination with green tea could potentially improve nutritional values on kombucha. This study aimed to evaluate the chemical and microbiological characteristics of kombucha made from robusta cascara and green tea. This study used Completely Randomized Design with one treatment factor namely combination of robusta cascara and green tea (100%:0%; 75%:25%; 50%:50%; 25%:75%; 0%:100%). The treatment repeated three times. The parameters observed were total polyphenol content, total titrated acid, total dissolved solid, pH, and total lactic acid bacteria. The result showed that combination of robusta cascara and green tea had significant effects on all parameters. The increased of green tea proportion in treatments could increase content of total titrated acid from 0.0032% (kombucha with 100% robusta cascara) to 0.0048% (kombucha with 100% green tea) whereas total dissolved solid from 0.0667% Brix to 9.1333% Brix and pH values from 3.6000 to 3.4667 slightly decreased in ombucha.The higher polyphenol content in the raw material the higher total polyphenol obtained in kombucha reflected on 100% green tea kombucha with 0.2245 mg GAE/mL. Total lactic acid bacteria of kombucha obtained at 3.3760 log CFU/mL to 4.3917 log CFU/mL.
Coffee and blood pressure
(Universidade Federal de Lavras, 2024-06-17) Nurvita, Silvia; Rizkaprilisa, Windy
Coffee has a characteristic taste, aroma and color that attracts its fans. Coffee is one of the drinks most consumed after mineral water. Annual coffee consumption is estimated at around 500 billion cups/year. Coffee contains a lot of caffeine and other chemical compounds. Coffee having a positive impact on health, also has a negative impact on health. The negative impact of coffee on health is that coffee affects blood pressure (BP) and the risk of hypertension. This research aims to determine the effect of coffee on blood pressure according to existing research. This research method uses the Literature Review (LR) research type. The article inclusion criteria used for this research were articles published in 2020-2023 with the keywords coffee, blood pressure, the relationship between coffee and blood pressure, the effect of coffee and blood pressure. This research found three articles that met the inclusion criteria for this research. The results of this research explain that three articles, there are two articles which prove that coffee consumption has no effect on blood pressure, but there is one article which shows that the duration of coffee consumption has an effect on systolic blood pressure. The conclusion of this literature study from these three articles can be concluded that on average coffee consumption has no effect on blood pressure. Coffee that is good for health is pure coffee that is brewed without a sugar.
Converting Arabica Coffee Parchment into value added products: Technical and Economic Assessment
(Universidade Federal de Lavras, 2024-07-29) Setiawan, Adi; Sitepu, Billy B.; Muhammad; Anshar, Khairul; Riskina, Shafira; Nurjannah, Siti; Hakim, Lukman
The coffee processing industry is experiencing a continuous rise in residues due to increased coffee-cherry production. However, the utilization of coffee parchment, which contains toxic compounds, remains limited and requires further investigation. This study aims to convert coffee parchment into biochar for potential use as a raw material for porous carbon material. The research was conducted using a purpose-built pilot-scale reactor. The goal was to address challenges related to operational cost, simplicity in operation, and maintenance, utilizing the Net Present Value (NPV) approach. Results indicated that coffee parchment comprised 34.5% biochar, 42.15% bio-oil, and balanced un-condensable-gas. Additionally, biochar products consisted of 42.02% fixed carbon and 38.63% volatile matter. The pyrolysis equipment designed for coffee parchment showcased economic viability, considering optimized annual operating days and scalability for production.
Converting Arabica Coffee Parchment into value added products: Technical and Economic Assessment
(Editora UFLA, 2024-07-29) Setiawan, Adi; Sitepu, Billy B.; Muhammad; Anshar, Khairul; Riskina, Shafira; Nurjannah, Siti; Hakim, Lukman
The coffee processing industry is experiencing a continuous rise in residues due to increased coffee-cherry production. However, the utilization of coffee parchment, which contains toxic compounds, remains limited and requires further investigation. This study aims to convert coffee parchment into biochar for potential use as a raw material for porous carbon material. The research was conducted using a purpose-built pilot-scale reactor. The goal was to address challenges related to operational cost, simplicity in operation, and maintenance, utilizing the Net Present Value (NPV) approach. Results indicated that coffee parchment comprised 34.5% biochar, 42.15% bio-oil, and balanced un-condensable-gas. Additionally, biochar products consisted of 42.02% fixed carbon and 38.63% volatile matter. The pyrolysis equipment designed for coffee parchment showcased economic viability, considering optimized annual operating days and scalability for production.
Coverage plants in coffee production systems as weed control
(Universidade Federal de Lavras, 2024-05-02) Barros, Vanessa Maria de Souza; Thimothee, Jean Alex; Rodrigues, Rafael Jorge Almeida; Gonçalves, Adenilson Henrique; Medeiros, Fernanda Carvalho Lopes de; Ferreira, André Dominghetti; Carvalho, Gladyston Rodrigues
The aim of this study was to evaluate the floristic and phytosociological compositions of weeds and the influence of cover crops between rows of organic and conventional coffee plantations. The assessment of weeds was carried out in two seasons (dry and rainy) in 2019 and 2020. A square made from welded iron bars of 0.50 x 0.50 m (0.25 m2) was launched four times in each block randomly, avoiding overlap, totaling 4.0 m2 of sampled area. A total of 41 weed species were found and described, which were distributed in 38 genera and 19 families with the predominance of Poaceae and Asteraceae. The most abundant species were Cyperus sp. and Urochloa decumbens Staf. that occurred simultaneously in all treatments and showed greater importance (IVI) among weeds. The similarity index is generally low, indicating that the weed community was affected by the presence and absence of cover crops.
Descriptive sensory tests for evaluating Coffea arabica: A systematic review
(Universidade Federal de Lavras, 2024-04-20) Nascimento, Manuella Oliveira; Ombredane, Alicia Simalie; Oliveira, Livia de Lacerda de
Coffee is a beverage whose price is closely related to the characteristics of its flavor, necessitating reliable sensory tests. To quantify their sensory attributes, classic sensorial methods such as Quantitative Descriptive Analysis (QDA) can be useful. However, uncertainties persist due to protocol variations, which made uncertain the quality of these protocols in evaluating coffee. This study aimed to conduct a systematic review to assess the quality of QDA protocols used for assessing Coffea arabica’s sensory attributes. The review encompassed various critical protocols control points, including pre-test procedures, coffee processing and preparation techniques, test application and data collection procedures. It was also summarized key attributes, highlighting factors impacting coffee’s sensory traits and bias risk of the studies. As the main results, it was saw that the studies have many limitations, such as not citing or controlling critical points in the tests procedures and application, which made most studies having a medium-high bias rating. The primary sensory results findings of the studies included topics such as the impact of brewing time, chemical compounds associated with sensory attributes and the effect of various roasting techniques on the sensory qualities of coffee. In conclusion, standardizing sensory evaluations in future research could enhance consistency and accuracy, yielding less biased results.
Development a novel and comprehensive cupping protocols for wine coffee assessment using Qualitative Description Analysis
(Editora UFLA, 2024-11-28) Hasni, Dian; Sulaiman, Ismail; Nazira, Mimi Misna
Diversification of coffee product and cnsumer demands attempt to create a new product such as wine coffee, a fermented coffee product which undergo the coffee cherries fermentation process up to 30 days or longer. As a new product, coffee wine does not have quality standard, which barriers the coffee trade and trust between coffee producers and buyer. This research aims to develop a novel and comprehensive cupping protocol for wine coffee using Qualitative Description Analysis with the presence of Specialty Coffee Association of America as guidelines. QDA is parted to three section panellist screening, panellist training and product evaluation. From 20 screened panellists, 12 are undergo to training panellist, but only 8 panelists shows consistent results, share similar understanding about wine coffee quality. The results showed that fragrance, aroma, flavour, body, aftertaste and acidity have different definition than specialty coffee from SCAA cupping protocols.
Diversity pattern on phytochemical traits of coffee bean skin from various species and harvesting methods
(Editora UFLA, 2024-11-12) Maxiselly, Yudithia; Bakti, Citra; Renaldi, Gerry; Sari, Dwi Novanda; Nusifera, Sosiawan; Maulana, Haris
Coffee is a well-known beverage consumed widely all around the globe. During its processing, a vast amount of waste was generated that can be utilized due to its bioactive compounds beneficial for human health. This study aimed to investigate the diversity pattern of three coffee pulp species based on phytochemical properties (total phenolic content (TPC), caffeine content, and antioxidant activity using the DPPH assay) exhibited by the skin of three coffee bean species: Coffea arabica (Arabica), Coffea canephora (Robusta), and Coffea liberica (Liberica) harvested with different technique (selective and strip picking). This study revealed that species and harvesting techniques significantly influenced the phytochemical properties of the coffee bean skin (CBS). The diversity pattern is shown by Principal Component Analysis (PCA), which has a correlation between Robusta and TPC and Arabica with Caffeine content. The findings from this study show the possibility of utilizing the waste from the coffee industry, especially the skin, to be used further in the development of products with considerable amounts of bioactive compounds.
Effect of coffee roasting on the cupping quality of coffee
(Universidade Federal de Lavras, 2024-05-08) Ting, Kuan-Fu; Chen, James C.; Chen, Tzu-Li
Coffee roasting is considered an important process that influences coffee cupping quality, nutrient composition, and consumer preference. Complex chemical reactions during the roasting process can change the chemical constituents of green coffee, thereby affecting its flavor and compositions. To the best of our knowledge, using the same roasting time, roasted bean color, or final roasting temperature but different time-temperature profiles will cause different cupping qualities. This study aimed to determine and quantify how the different roasting time-temperature profiles affect light-roasted coffee cupping qualities, such as flavor, aftertaste, acidity, body, balance, and overall. Herein, a rising (ROR) temperature rate between 150 °C and 185 °C was found to be the most important roasting stage affecting the cupping quality of light-roasted coffee. Moreover, the roast profile was not found to influence the coffee body during light roasting.
Effect of demucilagination and soaking in water with organic acids on the microbial, chemical, and sensory characteristics of coffee (Coffea arabica)
(Universidade Federal de Lavras, 2024-06-17) Ponce, Jorge Alfredo Cardona; Mejía, Luis Fernando Maldonado
There are divided opinions regarding sensory quality of mechanically demucilaginated (MD) coffee versus coffee that has undergone conventional fermentation (biological demucilagination). Fermenting and washing (wet process) requires high amounts of water that has contaminating effects upon its completion. Studies indicate that MD with soaking in organic acids could develop similar sensory quality to wet processed coffee. Organic acids are bioactive compounds that are naturally produced during conventional fermentation, which is why coffee has unique characteristics in the final cup profile. This study was conducted to assess the effects of soaking with organic acids (citric, ascorbic, and acetic) on the microbial, chemical, and sensory attributes of MD coffee. A Completely Randomized Design (CRD) was used, with a factorial arrangement (2×4+2) for a total of 10 treatments. The treatments were two soaking times (24 and 48 hours) and four soaking solutions (citric, ascorbic, acetic acid and water) in coffee with mechanical demucilagination, one treatment with mechanical demucilagination and one with fermentation and washing, both without soaking or acids. Microbiological counts of fungi, yeasts, and lactic acid bacteria (LAB) were carried out before and after soaking. Sensory characteristics were evaluated through cupping and chemical content and properties were studied by liquid chromatography and spectrophotometry. Microbial population demonstrated normal succession throughout the experiment with LAB been the most prevalent family during MD and fermentation. Soaking coffee in acid solutions maintained overall cupping scores with different attributes being detected by panelists. Phenolic compounds, caffeine and chlorogenic acids increased in soaked samples (acetic acid) but were similar to the control (fermentation). Flavonoid content ranged from 22 to 35 mg EC/g and was higher in samples soaked in acids compared to the controls. Green coffee extracts in general showed antioxidant activities greater than 80.9% comparable to other studies. Soaking time did not improve the quality characteristics of the coffee, but the type of acid used was able to modify the content and proportion of various families of chlorogenic acids in green coffee and total polyphenols, while maintaining sensory properties in comparison to fermented coffee.
Effect of demucilagination and soaking in water with organic acids on the microbial, chemical, and sensory characteristics of coffee (Coffea arabica)
(Editora UFLA, 2024-04-30) Ponce, Jorge Alfredo Cardona; Mejía, Luis Fernando Maldonado
There are divided opinions regarding sensory quality of mechanically demucilaginated (MD) coffee versus coffee that has undergone conventional fermentation (biological demucilagination). Fermenting and washing (wet process) requires high amounts of water that has contaminating effects upon its completion. Studies indicate that MD with soaking in organic acids could develop similar sensory quality to wet processed coffee. Organic acids are bioactive compounds that are naturally produced during conventional fermentation, which is why coffee has unique characteristics in the final cup profile. This study was conducted to assess the effects of soaking with organic acids (citric, ascorbic, and acetic) on the microbial, chemical, and sensory attributes of MD coffee. A Completely Randomized Design (CRD) was used, with a factorial arrangement (2×4+2) for a total of 10 treatments. The treatments were two soaking times (24 and 48 hours) and four soaking solutions (citric, ascorbic, acetic acid and water) in coffee with mechanical demucilagination, one treatment with mechanical demucilagination and one with fermentation and washing, both without soaking or acids. Microbiological counts of fungi, yeasts, and lactic acid bacteria (LAB) were carried out before and after soaking. Sensory characteristics were evaluated through cupping and chemical content and properties were studied by liquid chromatography and spectrophotometry. Microbial population demonstrated normal succession throughout the experiment with LAB been the most prevalent family during MD and fermentation. Soaking coffee in acid solutions maintained overall cupping scores with different attributes being detected by panelists. Phenolic compounds, caffeine and chlorogenic acids increased in soaked samples (acetic acid) but were similar to the control (fermentation). Flavonoid content ranged from 22 to 35 mg EC/g and was higher in samples soaked in acids compared to the controls. Green coffee extracts in general showed antioxidant activities greater than 80.9% comparable to other studies. Soaking time did not improve the quality characteristics of the coffee, but the type of acid used was able to modify the content and proportion of various families of chlorogenic acids in green coffee and total polyphenols, while maintaining sensory properties in comparison to fermented coffee.
Effect of extraction processing parameters using a ribbon blender on the physicochemical properties of coffee
(Universidade Federal de Lavras, 2024-07-03) Barriga, Paula Andrea Mayorga; Pardo, Ruth Yolanda Ruiz; Moreno, Fabian Leonardo Moreno
In this work, the effect of the water-coffee ratio, time, and stirring speed, on the total soluble solids, extraction rate, titratable acidity, and extraction yield was evaluated for the percolation of coffee in a horizontal ribbon blender equipment. The coffee extract was obtained in a pilot unit at a constant temperature of 85°C and varying the water-coffee ratio from 4:1 to 10:1; the stirring speed between 30 and 95 RPM and the extraction time from 10 to 60 minutes. It was determined that the water-coffee ratio was the factor that had a significant influence on all the response variables, while time and stirring were significant for the extraction rate and titratable acidity. The optimal setup among the studied conditions was using a water temperature of 85°C, a water-coffee ratio of 4:1, and a stirring speed of 66 RPM for ten minutes. At these conditions, an extract of 5.85% TDS, 14.54% as yield, an extraction rate of 654.8 g/h, and a content of 5.62 mg of CGA/mL was obtained. Hence, this study presents an alternative process to obtain coffee extract in producing soluble coffee at a low industrial scale.
Effect of storage duration on phenolics stability in ready-to-drink coffee beverage
(Universidade Federal de Lavras, 2024-06-20) Herawati, Dian; Davin, Christophorus; Yuliana, Nancy Dewi; Yulianti, Yulianti
Ready-to-drink (RTD) coffee is one of the innovations in coffee beverages that is widely circulated in the market and is quite popular among various groups of people. This study aimed to partially validate the Folin-Ciocalteu method for phenolic compounds analysis in ready-to-drink (RTD) coffee and determine the effect of storage time on the stability of phenolic compounds in RTD coffee. Coffee drinks were made from Robusta coffee extract added with other ingredients (sugar and potassium sorbate), pasteurized, hot filled into plastic cups, and stored at room temperature (25 – 28 oC) for 0, 2, 4, 6, and 8 weeks. Two proposed methods (Folin-Ciocalteu methods) for routine phenolic analysis (Total phenolic content (TPC) and total tannin content (TTC)) in RTD coffee were partially validated. In general, partial validation parameters of TPC gave more satisfying results, such as linearity (R2 = 0.9991); precision (RDS < 2/3 RSDHorwitz); recovery (109%) and sensitivity (LOD = 14.20 mg L-1). Phenolic stability in RTD coffee was evaluated using the Folin-Ciocalteu method (TPC and TTC) and HPLC method (individual caffeoylquinic acids (CQAs) (3-CQA, 4-CQA, and 5-CQA)). In general, TTC values in all storage time were higher than TPC values because the TTC method had higher recovery (132%). Furthermore, the three parameters observed (TPC, TTC and CQAs) experienced significant degradation during storage. The decrease percentage of TPC and TTC during 8 weeks of storage was 11% (from 63 to 55 mg/100 mL) and 10% (160 to 143 mg/100 mL), respectively. Meanwhile, 5-CQA was the most sensitive CQAs and its degradation for 8 weeks of storage reached 19% (from 28 to 23 mg/100mL ). TPC is recommended for monitoring the effect of storage duration on phenolic compound stability in RTD coffee and analysis of 5-CQA as the most unstable individual phenolic may support the monitoring.
Effect of temperature and photoperiod on Pseudomonas syringae pv. garcae inoculum production
(Editora UFLA, 2024-11-12) Oliveira, Júlia Marques; Pozza, Edson Ampélio; Belan, Leônidas Leoni; Freitas, Marcelo Loran de Oliveira; Tedardi, Vitória Moreno
Bacterial halo blight (BHB), whose etiological agent is the bacterium Pseudomonas syringae pv. garcae, is one of the main diseases occurring in coffee (Coffea arabica L.) leading to losses in nurseries and fields. This study aimed to assess the effect of five temperatures (20, 25, 30, 35, and 40 °C) and three photoperiods (continuous light, 12-hour light/dark, continuous dark) on the P. syringae pv. garcae inoculum concentration and the BHB progression re sulted from this inoculum, in coffee seedlings. Using a factorial design (5 x 3) with four replicates, Petri dishes inoculated with P. syringae pv. garcae were incubated for 48 hours, and the concentrations and bacterial cell dimensions were measured. The resulting inoculum was then used to inoculate coffee seedlings at 23 ± 2 °C in one leaf pair by wounding inoculation using a multi-needle apparatus. The disease severity was assessed, and the area under the disease progress curve (AUDPC) was calculated. Significant interaction (p < 0.05) between temperature and photoperiod was observed for inoculum concentration and cell dimensions. The highest inoculum concentration (2.2 x 10⁹ CFU/ml) was recorded at 25 °C under continuous light. The largest bacterial cells (4.4 µm in length and 0.7 µm in width) resulted from temperatures of 30 °C with 12-hour light/dark. For the AUDPC of BHB, a difference (p < 0.05) was observed only between inoculum production temperatures. The highest AUDPC was recorded at 25 °C, with a gradual decline observed as the temperature increased.
Enzymes activity as potential molecular markers for drying tolerance and quality in Coffea arabica seeds
(Editora UFLA, 2024-09-19) Freitas, Aline Silva; Pinho, Édila Vilela de Resende Von Pinho; Santos, Heloisa Oliveira dos; Pereira, Elise de Matos; Pereira, Wilson Vicente Souza
Antioxidant enzyme expression plays a vital role in the stress response in plants. This study evaluated the physiological quality and antioxidant enzyme expression of coffee seeds and plants subjected to drying. Coffee seeds were conventionally dried to 40% and 10% water content, representing nonstressed and stressed conditions, respectively. The tests included germination, accelerated aging, and emergence. The expression of enzymes (SOD, CAT, POX, APX, and 1-cys-prx), together with lipid peroxidation, was analysed. The results showed no significant differences in the physiological test results, except for germination in stressed seeds. We found differences in antioxidant system enzymes according to the condition applied to the seeds; however, only for peroxidase, lipid peroxidation, and 1-cys-prx were the observed patterns more strongly correlated with seed germination. Nevertheless, only lipid peroxidation and 1-cys-prx were more strongly correlated with germination, indicating its potential as a marker for coffee seed quality.
Evaluation of Lactobacillus Coagulans in the design of coffee-based probiotic beverages by using different preparation methods
(Universidade Federal de Lavras, 2024-06-13) Ormaza-Zapata, Angela María; Barona, neyder Rodríguez; Arango, Félix Octavio Díaz
The purpose of the presented work was to assess the viability of microcapsules containing Bacillus coagulans. These microcapsules were produced through spray drying and employed two different combinations of wall materials. The evaluation was carried out within the context of creating coffee-based beverages using nine distinct preparation methods. Various characteristics of the microcapsules were measured, including solubility, wettability, hygroscopicity, moisture content, and water activity. Sensory analysis was used to determine the proportion of probiotic incorporation in the roasted and ground coffee. Moreover, the viability and count of probiotics were quantified in the nine preparations, both in cold and hot water. Among the preparation methods, the Chemex approach demonstrated enhanced viability when utilized for hot water preparation, while the Cold Tower method excelled in cold water scenarios. The outcomes of the study underscore the considerable potential of Bacillus coagulans in developing coffee-based probiotic beverages. These beverages exhibit noteworthy chemical, sensory, and functional stability.