Coffee Science
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Item The application of arbuscular mycorrhizal fungi and mycorrhizae helper bacteria combined with compost to reduce the Pratylenchus coffeae population in an Arabica coffee plantation(Editora UFLA, 2024-10-31) Hindersah, Reginawanti; Asyiah, Iis NurArbuscular mycorrhizal fungi (AMF) and mycorrhiza helper bacteria (MHB) are potential biological agents that control Pratylenchus coffeae, a major endoparasitic nematode in coffee plantations. An experiment was conducted to determine the role of AMF and MHB inoculants combined with compost in reducing plant damage and the number of nematodes in the soil and roots, as well as increasing the AMF infection rate in juvenile (JP) and mature (MP) Arabica coffee plants. The field experiment with a randomized block design was conducted with both juvenile (JP) and mature plants, consisting of 8 combination treatments and one control with three replications. The JP and MP plants received 1.5-50 g and 25-100 g AMF inoculant/plant and compost at doses of 5 or 7.5 and 10 or 15 kg/plant, respectively, while the MHB liquid inoculant concentrations were 10 mL and 100 mL, respectively. The control plants were not treated with AMF, MHB, or compost. The AMF and MHB inoculants combined with compost reduced leaf and root damage, but increased the degree of AMF infection in the roots of JP and MP. The nematode populations in roots and shoots of treated JP were reduced by 48.7-74.5% and 56.0 80.5%, respectively; in mature plants, the reductions ranged from 40.5 to 74.4% in roots and 44.0 to 75.4% in the soil. These results suggest that AMF, MHB, and compost effectively reduced P. coffeae infection in Arabica coffee plants under field conditions.Item Free radical scavenging activity-based antioxidant analysis and infrared spectroscopy of peppermint-flavored coffee drink(Editora UFLA, 2024-12-05) Kustyawati, Maria Erna; Nurdin, Samsu Udayana; Thaliarinanta, Angela SarazPeppermint essential oil canserve as a healthy flavoring additive to coffee drinks and provide health benefits by enhancing their antioxidant activity. Hence, this study aimed to examine the antioxidant activity of peppermint-flavored coffee drinks using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2’-azinobis-(3-ethylenebenzothiazoline)-6-sulfonic acid (ABTS) free radical scavenging assays. Furthermore, Fourier transform infrared-attenuated total reflectance spectroscopy was performed to investigate the profile of compounds contributing to the antioxidant property. The experiments were conducted using a Complete Randomized Block Design with six replications. Two-factor treatments were as follows: soaking time (S), 0 (S0), 5 (S1), and 10 (S2) min; peppermint essential oil concentration (E), 0 (E0), 0.5 (E1), and 0.75% (E2) (v/v). N otably, both DPPH and ABTS assays revealed increased antioxidant activity of peppermint-flavored coffee drink where the coffee was soaked for 10 min in 0.75% peppermint solution. The coffee soaked for 10 min in 0.5% and 0.75% peppermint solutions showed a high scavenging effect against ABTS free radical, with 93.73 and 94.30% inhibition activity and half-maximal inhibitory concentrations of 0.272 and 0.267 ppm, respectively. Additionally, a new peak was observed at 1640 cm−1in peppermint solution-soaked coffee beans,which corresponded tothe C=C functional group in the limonene spectrum, an antioxidant compound in peppermint. Altogether, the findings show that peppermint essential oil enhanced the antioxidant activity of the coffee.Item Characterization in populations of Coffea arabica L. for resistance to CBD using molecular markers(Editora UFLA, 2024-09-24) López-Monsalve, Luisa F.; Quiroga-Cardona, Julio; López, Natalia Arango; Ramírez-Cardona, Carlos A.; Flórez-Ramos, Claudia P.Coffee berry disease (CBD) is caused by the fungus Colletotrichum kahawae Waller and Bridge. This disease is restricted to the African continent, where it can cause production losses of more than 80% when susceptible varieties are used or when the indicated chemical control is not carried out. For this reason, since 1970, Cenicafé has developed lines resistant to this disease in the absence of the pathogen, a process that has been favored by the discovery and validation of microsatellite markers associated with the Ck-1 gene for resistance to CBD. In this research, 12 populations of Coffea arabica were characterized for their resistance to CBD using the molecular markers Sat235, Sat207 and FR34-6CTG. The molecular markers allowed us to identify that the same allelic form of resistance to CBD is present in lines derived from Timor Hybrid CIFC 1343 (HdT CIFC 1343). Furthermore, the allelic form of resistance associated with the three molecular markers was identified in one line derived from Coffea canephora. In lines derived from Caturra x HdT CIFC 1343 it was evident that, when the plants present the allelic forms of resistance identified by the molecular markers, high percentages of hypocotyls resistant to different isolates of C. kahawae are observed in the progeny.Item Physicochemical and sensory analysis of coffee: A determination in different parts of the plant(Editora UFLA, 2024-07-29) Sousa, Rachel Machado de; Paiva, Leandro CarlosThe quality of the Arabica coffee drink (Coffea arábica L) is the result of the interaction of several factors, such as climatic conditions, terroir, altitude, nutritional factor, management aspects, stage of fruit maturation, harvest, among others. The objective was to evaluate the physical-chemical quality of coffee fruits, through the soluble solids content of coffee beans, in different parts of the plant, in order to define which position/location on the plant presents the best results in relation to sensory attributes of the drink. The analysis of soluble solids (SS) in ºBrix of cherry and raw coffee, mass grains (MMG) and sensory evaluation, the Catuaí IAC 144 variety presented better results in relation to the Paraíso MG 419- 1 variety. Regarding electrical conductivity (EC), total titratable acidity (TTA) of raw coffee, pH, color the results of the two varieties were representative for quality, the values found in the analyzes are within the defined ranges. The experimental design used was randomized blocks and each variety was harvested in three blocks. Each block consisted of a plant, each plant was divided into twelve subdivisions. The treatments were arranged in a 12x2x3 factorial scheme and subjected to analysis of variance (ANOVA) and the means compared using the Scott Knott post hoc test with a significance level (p-value ≤ 0.05). The correlation matrix was used between the variables under study, in order to verify whether there is a linear relationship or not between the variable’s soluble solids ºBrix of cherry and raw coffee, aroma and body of the drink with the final average score of the tasters. Interpreting the generated equation, there is an association between body and final score, when increasing one unit in the body, an increase of 2.21 in the final score is expected. The positions on the coffee plant, upper third and lower third, in relation to the varieties Catuaí IAC 144 and Paraíso MG 419-1, were the most promising from the perspective of the results found in the physical-chemical and sensorial analysis.Item Sensory evaluation and color of Coffea arabica coffee from the Central Mexican Highlands(Editora UFLA, 2024-11-12) Flores, Omar Roberto Vargas; Arriaga, Martín Rubí; Berasain, María Dolores Mariezcurrena; Elías, Guillermo Asdrúb al Vargas; Dávila, José Francisco RamírezCoffee is cultivate d worldwide at an average altitude of 1,500 m worldwide, but in Mexico is typically cultivated around 1,200 m. However, in Temas caltepec, Mexico State, coffee is grownat 2,100 m, resulting in a slower development process that yields a “differentiated quality.” This quality has been nationally recognized atthe “Cup of Excellence” competition, where coffees scoring above ninety points out of a hundred have received the “presidential award.” Thisrecognition has enabled these coffees to be sold as “specialty” coffeeat auction to Europe and Asia, fetching higher prices per pound and thus improving economic returns. In this study, wescientifically validate these claims by evaluating sensory quality in the cup and color ofthe bean. , for which two toasting sta rt temperatures were used. The color in roasted coffee is derived from caramelization due to the high temperatures to which it is subject ed during this process. This characteristic has an intrinsic influence for the taster even though it is not objectively measured during the cupping process. For this reason, it was decided to carry out the measurement in this research. In 2022, Arabica coffee beans of the Caturra variety were harvested from Temascaltepec using a natural process. The coffee was roasted according to the Specialty Coffee Association (SCA) protocol at five time intervals (8, 9, 10, 11, and 12 min) starting fromtwo initial roasting temperatures (210 and 215 °C). The roasted coffee was evaluated using thesensory analysis protocol of the SCA, which assessed fragrance/aroma; flavor, aftertaste, acidity, body, and balance; sweetness, uniformity, and cleanliness. In addition, we quantified the color of the coffee bean using a colorimeter. Sensory evaluation indicated that the best treatment was 215 °C/10min, with a predominant vanilla aroma (balsamic-sweet aromatic family) and an L* value of 13, suggesting a medium roast that may retain its functional properties. All treatments yielded values within proposed standards for a medium roast.Item Risk identification of supply chain system in coffee processor: A case of Indonesia(Editora UFLA, 2024-11-26) Suryaningrat, Ida Bagus; Hapsari, Shinta Syafrina EndahCoffee is one of Indonesia's top agricultural products, earning US$815,933,000 on an export basis, with 8.93% (64,804 tons) of the production coming from East Java (Indonesian Central Bureau of Statistics, 2019). Coffee processors face many uncertain conditions in the supply chain, which are commonly referred to as risks. Risk management is important in any business process to optimize, monitor, evaluate and maintain the continuity of business strategy and resources. This research aims to analyze and mitigate risks in the coffee processor's supply chain system. Risk identification and analysis, aggregate risk potential (ARP) score, risk evaluation and treatment, and effective to difficulty (ETD) score were implemented in this study. This study found that the risk drivers have the most significant relationship with the 28 listed risk events. On the other hand, 10 mitigation strategies have a strong relationship with these risk agents and have high effectiveness and lower difficulty.Item Effect of temperature and photoperiod on Pseudomonas syringae pv. garcae inoculum production(Editora UFLA, 2024-11-12) Oliveira, Júlia Marques; Pozza, Edson Ampélio; Belan, Leônidas Leoni; Freitas, Marcelo Loran de Oliveira; Tedardi, Vitória MorenoBacterial halo blight (BHB), whose etiological agent is the bacterium Pseudomonas syringae pv. garcae, is one of the main diseases occurring in coffee (Coffea arabica L.) leading to losses in nurseries and fields. This study aimed to assess the effect of five temperatures (20, 25, 30, 35, and 40 °C) and three photoperiods (continuous light, 12-hour light/dark, continuous dark) on the P. syringae pv. garcae inoculum concentration and the BHB progression re sulted from this inoculum, in coffee seedlings. Using a factorial design (5 x 3) with four replicates, Petri dishes inoculated with P. syringae pv. garcae were incubated for 48 hours, and the concentrations and bacterial cell dimensions were measured. The resulting inoculum was then used to inoculate coffee seedlings at 23 ± 2 °C in one leaf pair by wounding inoculation using a multi-needle apparatus. The disease severity was assessed, and the area under the disease progress curve (AUDPC) was calculated. Significant interaction (p < 0.05) between temperature and photoperiod was observed for inoculum concentration and cell dimensions. The highest inoculum concentration (2.2 x 10⁹ CFU/ml) was recorded at 25 °C under continuous light. The largest bacterial cells (4.4 µm in length and 0.7 µm in width) resulted from temperatures of 30 °C with 12-hour light/dark. For the AUDPC of BHB, a difference (p < 0.05) was observed only between inoculum production temperatures. The highest AUDPC was recorded at 25 °C, with a gradual decline observed as the temperature increased.Item Development a novel and comprehensive cupping protocols for wine coffee assessment using Qualitative Description Analysis(Editora UFLA, 2024-11-28) Hasni, Dian; Sulaiman, Ismail; Nazira, Mimi MisnaDiversification of coffee product and cnsumer demands attempt to create a new product such as wine coffee, a fermented coffee product which undergo the coffee cherries fermentation process up to 30 days or longer. As a new product, coffee wine does not have quality standard, which barriers the coffee trade and trust between coffee producers and buyer. This research aims to develop a novel and comprehensive cupping protocol for wine coffee using Qualitative Description Analysis with the presence of Specialty Coffee Association of America as guidelines. QDA is parted to three section panellist screening, panellist training and product evaluation. From 20 screened panellists, 12 are undergo to training panellist, but only 8 panelists shows consistent results, share similar understanding about wine coffee quality. The results showed that fragrance, aroma, flavour, body, aftertaste and acidity have different definition than specialty coffee from SCAA cupping protocols.Item Performance of Chitosan Beads after treatment with spent coffee grounds for the adsorption of methylene blue(Editora UFLA, 2024-10-11) Nugraheni, Ari Dwi; Nurmayasari; SholihunSpent coffee grounds are one of the ingredients that are abundantly available today and can be used as a crosslinking material with other materials. The objective of this work was chitosan (CS) beads and treatment spent coffee grounds (TCG) made into beads to determine their ability to adsorb methylene blue (MB). The ratios of CS to TCG (by weight) were varied to make beads with the following composition: 1:0 (CS), 1:1 (CS/TCG 3%), 1:2 (CS/TCG 6%), and 1:3 (CS/TCG 9%). Glutaraldehyde crosslinking was performed to increase the stability of the beads in water; these beads were used to test the capacity to adsorb MB. The beads of different compositions were assessed using several techniques, including scanning electron microscopy (SEM), Fourier-transform infrared (FTIR) and ultraviolet-visible spectroscopy (UV-Vis). The results of the morphological analysis showed that adding TCG resulted in the formation of more pores and cavities in each sample. It enhances its effectiveness as an adsorbent. Analysis of the composition of TCG via XRF showed the presence of a carbon atom, a precursor of activated carbon. The FTIR spectrum indicated that functional groups played a crucial role in adsorption. The UV-Vis results showed that the degradation percentage increased with an increase in the weight ratio of CS/TCG beads. The CS beads treated with TCG could be used to adsorb MB for up to seven repetitions, with an adsorption percentage of 87.8%.Therefore, it is concluded that the measured CS/TCG data were numerically fitted using Langmuir and Freundlich models to analyze the adsorption isotherm behavior.Item Sensory evaluation of Poncokusumo Liberica Coffee based on bean size and post-harvest processing(Editora UFLA, 2024-09-19) Wafaretta, Erona; Jatmiko, Yoga Dwi; Sunarharum, Wenny Bekti; Hakim, LuchmanCoffea liberica from local farm in Poncokusumo, Indonesia has several accessions identified, unfortunately there has been no further sensory research to develop it into high quality coffee products. This study was aimed to evaluate the sensory attributes of different liberica coffee accessions from a local farm in Poncokusumo based on beans size and post-harvest processes. Liberica coffee green beans were dried using two processes: natural and wine, followed by measuring its bean size. The beans were roasted using three roasting levels (light, medium, and dark), and sensory attributes were tested using a cupping test with nine trained panelists based on Specialty Coffee Association (SCA) guidelines. The findings show that the bean size and post-harvesting processes affected the coffee’s sensory quality. The highest total score was 77.69 for beans that were dried by natural process and roasted at a light level. Compared to wine processing which earns a “good” score, the optimal drying technique for liberica coffee beans that receive a “very good” grade was natural processing. The flavor of liberica coffee was most optimal at light to medium roasting levels, which produced “very good” results that differed from dark levels. The link between bean size, post-harvest, and sensory attribute values demonstrates that the size and procedure of coffee processing is an important sequence to be observed in order to improve the result to the highest quality. It is expected that the findings of this study can be applied to examine the quality standards of local liberica coffee products in Poncokusumo.