Coffee Science

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    Effect of temperature and photoperiod on Pseudomonas syringae pv. garcae inoculum production
    (Editora UFLA, 2024-11-12) Oliveira, Júlia Marques; Pozza, Edson Ampélio; Belan, Leônidas Leoni; Freitas, Marcelo Loran de Oliveira; Tedardi, Vitória Moreno
    Bacterial halo blight (BHB), whose etiological agent is the bacterium Pseudomonas syringae pv. garcae, is one of the main diseases occurring in coffee (Coffea arabica L.) leading to losses in nurseries and fields. This study aimed to assess the effect of five temperatures (20, 25, 30, 35, and 40 °C) and three photoperiods (continuous light, 12-hour light/dark, continuous dark) on the P. syringae pv. garcae inoculum concentration and the BHB progression re sulted from this inoculum, in coffee seedlings. Using a factorial design (5 x 3) with four replicates, Petri dishes inoculated with P. syringae pv. garcae were incubated for 48 hours, and the concentrations and bacterial cell dimensions were measured. The resulting inoculum was then used to inoculate coffee seedlings at 23 ± 2 °C in one leaf pair by wounding inoculation using a multi-needle apparatus. The disease severity was assessed, and the area under the disease progress curve (AUDPC) was calculated. Significant interaction (p < 0.05) between temperature and photoperiod was observed for inoculum concentration and cell dimensions. The highest inoculum concentration (2.2 x 10⁹ CFU/ml) was recorded at 25 °C under continuous light. The largest bacterial cells (4.4 µm in length and 0.7 µm in width) resulted from temperatures of 30 °C with 12-hour light/dark. For the AUDPC of BHB, a difference (p < 0.05) was observed only between inoculum production temperatures. The highest AUDPC was recorded at 25 °C, with a gradual decline observed as the temperature increased.
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    Initial stages of infectious process of Pseudomonas syringae pv. garcae in coffee leaves
    (Editora UFLA, 2019-07) Souza, José Otávio Gusmão de; Pozza, Edson Ampélio; Belan, Leônidas Leoni; Freitas, Marcelo Loran de Oliveira; Oliveira, Júlia Marques
    Bacterial blight of coffee (Pseudomonas syringae pv. garcae) (PSG) has been causing damage and losses to coffee growing. However, initial stages of the infectious process should be described in order to understand the relationship cycle and to propose efficient management strategies. This study described the initial stages of infectious process of the PSG in coffee leaves over time. The bacterial penetration sites studied were stomata, leaf margins, and wounds. Coffee seedlings received 50μL inoculum suspension at marked points on the abaxial face and leaf margins, and around wounds. By 30 minutes, 1, 3, 6, and 12 hours after inoculation, leaf disks collected at inoculated points were analyzed with scanning electron microscopy. One hour after inoculation, bacterial groups could be seen as result of cell multiplication. Subsequently, the number of grouped bacteria increased proportionally over time. A growing population concentration was found near stomata, wounds, epidermal appendages, and wax layer on leaf margins and leaf tip. From these places bacteria were positioned in their long axis starting from structures, thus created a population gradient and displacement flow toward penetration sites. Initial stages of infectious process occurred in all penetration sites, thus it was not possible to determine a preferred pathway.