Bragantia
URI permanente para esta coleção${dspace.url}/handle/123456789/9887
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Item Large-scale gene expression analysis reveals the role of primary metabolism regulation in resistance to Brazilian Pseudomonas coronafaciens pv. garcae in coffee(Instituto Agronômico (IAC), 2024-12-20) Schenk, Juliana Camargo Martinati; Rodrigues, Lucas Mateus Rivero; Arruda, Natália; Guimarães, Paula de Souza; Diniz, Leandro Cardamone; Rezende, Antonio Mauro; Destéfano, Suzete Aparecida Lanza; Padilha, Lilian; Maluf, Mirian Perez; Guerreiro Filho, OliveiroThis study investigated the response of arabica coffee plants to the pathogen Pseudomonas coronafaciens pv. garcae using RNA-seq technology. Susceptible and resistant coffee plants were inoculated with the bacteria, and leaf samples were collected at different time points for RNA sequencing. Seven genes related to different defense pathways were chosen for expression quantification in time-course experiments using infected leaves from resistant and susceptible plants, as well as non-infiltrated and water-infiltrated leaves as controls. The results obtained revealed that response mechanisms differ between genotypes and provide insights into the genetic basis of early defense in coffee plants against P. coronafaciens pv. garcae, offering potential strategies for genetic breeding.Item Bacterial halo blight of coffee crop: aggressiveness and genetic diversity of strains(Instituto Agronômico (IAC), 2018-01) Maciel, Karen Wolf; Destefano, Suzete Aparecida Lanza; Beriam, Luis Otavio Saggion; Almeida, Irene Maria Gatti de; Patricio, Flavia Rodrigues Alves; Rodrigues, Lucas Mateus Rivero; Guerreiro Filho, OliveiroBacterial halo blight, caused by Pseudomonas syringae pv. garcae, is an important disease of coffee crop occurring in Brazil and other countries. In recent years, outbreaks of this disease have damaged several coffee crops in Brazil. Aggressiveness and genetic diversity of 25 strains of P. s. pv. garcae, obtained between the years 1958 and 2011, in 23 cities of São Paulo and Minas Gerais states, as well as three strains from Kenya were evaluated in this study. The strains were inoculated on coffee seedlings cultivar Mundo Novo, and their genetic diversity was evaluated by ERIC-PCR, REP-PCR, and their combination. All the strains were pathogenic to the coffee seedlings; the results of pathogenicity tests, in both experiments, could be divided in four aggressiviness classes (highly aggressive; aggressive; moderately aggressive and less aggressive). The Kenyan strains grouped separately from the Brazilian strains with ERIC-PCR and the combination of ERIC- and REP-PCR. The Brazilian strains could be grouped in two sub-clusters, the first including the older strains, obtained from 1958 to 1978, and the other comprising the remaining strains. With a few exceptions, strains isolated from 1997 to 2011, grouped mainly by their region of origin, were predominantly isolated from higher altitude regions, above 800 m. This probably occurred because the climatic conditions that prevail in these regions, characterized by milder temperatures and regular rainfall, are favorable for the coffee crop and for the production of high quality coffee beverage, but can be also favorable to bacterial halo blight.