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Item Large-scale gene expression analysis reveals the role of primary metabolism regulation in resistance to Brazilian Pseudomonas coronafaciens pv. garcae in coffee(Instituto Agronômico (IAC), 2024-12-20) Schenk, Juliana Camargo Martinati; Rodrigues, Lucas Mateus Rivero; Arruda, Natália; Guimarães, Paula de Souza; Diniz, Leandro Cardamone; Rezende, Antonio Mauro; Destéfano, Suzete Aparecida Lanza; Padilha, Lilian; Maluf, Mirian Perez; Guerreiro Filho, OliveiroThis study investigated the response of arabica coffee plants to the pathogen Pseudomonas coronafaciens pv. garcae using RNA-seq technology. Susceptible and resistant coffee plants were inoculated with the bacteria, and leaf samples were collected at different time points for RNA sequencing. Seven genes related to different defense pathways were chosen for expression quantification in time-course experiments using infected leaves from resistant and susceptible plants, as well as non-infiltrated and water-infiltrated leaves as controls. The results obtained revealed that response mechanisms differ between genotypes and provide insights into the genetic basis of early defense in coffee plants against P. coronafaciens pv. garcae, offering potential strategies for genetic breeding.Item Sample size estimation of fruit maturation for Arabica’s coffee(Instituto Agronômico (IAC), 2025-01-31) Botega, Gustavo Pucci; Abrahão, Juliana Costa de Rezende; Botelho, Thiago Tavares; Botelho, Cesar Elias; Salvador, Guilherme Soares; Gonçalves, Flávia Maria AvelarThis study aimed at establishing the ideal sample size for evaluating the maturation cycle in Coffea arabica, and investigating the errors associated with different sample sizes, in addition to verifying the possibility of using the clustering method to separate genotypes according to the maturation stage. Two experiments were analyzed: one with F2:3 progenies using visual maturation assessment through fruit counting, and another with cultivars using image processing for maturation assessment. To determine the ideal sample size for this trait, we used the estimation of the errors associated with maturation, using the bootstrap technique. Subsequently, the K-means algorithm was tested as an alternative for clustering genotypes into maturation classes. The application of the bootstrap technique in order to estimate the error associated with maturation revealed that the adoption of a 450-mL sample size resulted in an associated error of approximately 5%, indicating that it is an adequate size for character assessment. The implementation of K-means as a clustering tool offers a promising perspective for Arabica coffee plant breeding programs. A more comprehensive analysis, which not only assesses the proportion of ripe fruits, but also considers the distribution of different maturation stages, provides a more accurate understanding of the maturation process. This allows a more precise identification of genotypes with the most suitable performance for different growing conditions, as well as enabling adjustments in harvest management and post-harvest processing, optimizing coffee quality.Item Contribution of mycorrhiza and phosphate-solubilizing fungi in coffee seedling growth in four soils with different fertility conditions(Instituto Agronômico (IAC), 2025-01-17) González-Osorio, Hernan; Sadeghian, Siavosh; Mira, Beatriz EugeniaPhosphorus (P) is a limiting nutrient for coffee seedlings. To meet this requirement, coffee growers usually apply di-ammonium phosphate (DAP), which is highly sensitive to local price fluctuations. The use of arbuscular mycorrhizal fungi (AMF) and phosphate-solubilizing fungi (PSF) has been recommended, but the results derived from their application are inconsistent and uncertain, depending on soil fertility status. The response of AMF and PSF application in the plant growth of coffee seedlings during the nursery stage was evaluated in four non-sterile soils, which represent the most abundant type of soils in the coffee regions of Colombia: Eutropept, Dystropept, Fulvudand, and Ultisol. The treatments included a commercial AMF inoculum, PSF-Phlebia subserialis-CH4, and a consortium of AMF+PSF, at four P levels (0, 0.5, 1, and 2 g.plant-1 P2O5) using DAP fertilizer. Shoot dry weight (SDW) was affected by P applications in Fulvudand. In the other soils, a P-response occurred with 0.5 g.plant-1 , whereas 1 and 2 g.plant-1 of P2O5 caused a significant reduction in SDW. AMF increased SDW by 40% in Eutropept. The negative effect of higher P amounts was mitigated (> 70%) through PSF and AMF+PSF in Ultisol and Eutropept, respectively. In plants in which microorganisms increased SDW, the P concentration was 0.17 to 0.22%. The plant growth promotion of coffee seedlings during the nursery stage with AMF and/or PSF applications varied according to soil type and P amounts applied.Item Potential of new Coffea arabica cultivars for renewal of Meloidogyne paranaensis infested crop(Instituto Agronômico (IAC), 2025-06-09) Luz, Silvana Ramlow Otto Teixeira da; Teixeira, Lívia Pimenta; Salgado, Sonia Maria de Lima; Andrade, Vinícius Teixeira; Marques, Elizabeth Rosemeire; Botelho, Cesar Elias; Fatobene, Bárbhara Joana dos Reis; Carvalho, Gladyston RodriguesMeloidogyne paranaensis is one of the most damaging species of root-knot nematode to coffee trees. The development of resistant cultivars is crucial to the continuity of cultivation in infested areas. Thus, the aims of this study were to assess the performance of F6:7 progenies derived from the Amphillo germplasm in an infested area and to validate the new Coffea arabica MGS Vereda and MGS Guaiçara cultivars. The Catuaí Amarelo IAC 62 cultivar was used as the susceptible standard, and IPR 100 as the resistance standard. The experiment was conducted in 2018 using a randomized complete block design with four replications and 15 plants per plot. Resistance related and agronomic traits were assessed over four years. The lowest population of M. paranaensis was observed in progenies 88, 44B, and 105 from MGS Guaiçara, MGS Vereda, and IPR 100 cultivars, respectively. The progenies with the lowest population of M. paranaensis, although resistant, were not productive. The new MGS Vereda cultivar stood out in terms of yield and early fruit ripening, with the highest proportion of cherry fruit at harvest and a low incidence of peaberry grain. The results suggest MGS Vereda cultivar’s potential for the renewal of coffee cultivations occurs in a rainfed system according to the environmental conditions of the experiment.Item Coffee industry waste-derived biochar: characterization and agricultural use evaluation according to Brazilian legislation(Instituto Agronômico (IAC), 2021-09-13) Carnier, Ruan; Coscione, Aline Renee; Delaqua, Douglas; Abreu, Cleide Aparecida deThe agricultural use of biochar has been the focus of much research in the last decade due to the improvement of soil chemical, physical, and biological attributes. Nonetheless, Brazil still has no specific legislation for biochar, limiting its agricultural use. The objective of the present work is to evaluate the use of biochar produced from spent coffee grounds (SCG) and coffee parchment (CP) by slow pyrolysis at 700 °C according to the existing framework of the Brazilian Ministry of Agriculture, Livestock, and Food Supply (MAPA) legislation for organic fertilizer, soil conditioner or plant substrate. Biochar was characterized according to normative instructions No. 17, 31, 61, 7, 5 and 35. Although not required by the addressed legislation, the semitotal content of macro- and micronutrients was also determined. While CP biochar could be used as an organic fertilizer or plant substrate, SCG biochar, due to its higher Ni content and lower than required cation exchange capacity (CEC), did not meet MAPA legislation criteria to allow for its agricultural use. Future regulations can be based on the current standards, and structural attributes, such as total C content, particle size distribution, and complete macro- and micronutrient determination should be included. Further research may also indicate the viability of biochar use as a soil conditioner based on a more representative set of biomasses with a higher CEC. These considerations will help to take advantage of the benefits of biochar to soil, contributing to a circular economy, which is still at a difficult stage in Brazil.Item Physiological quality of seeds of Coffea canephora from early and late clones during maturation(Instituto Agronômico (IAC), 2024-03-21) Crasque, Jeane; Brandão, Thielen Martins dos Santos; Cerri Neto, Basílio; Comério, Marcone; Volpi, Paulo Sérgio; Arantes, Lúcio de Oliveira; Machado Filho, José Altino; Milanez, Camilla Rozindo Dias; Dousseau-Arantes, SaraThe objective of this study was to evaluate the physiological quality of seeds from early and late maturing Coffea canephora clones, aiming to identify the ideal harvest time. The fruits were collected every two weeks from 188 days after anthesis (DAA) and characterized as green, cane green, cherry, raisin and dry. The seeds were evaluated for water content, germination percentage, normal and abnormal seedlings, nongerminated seeds and dead seeds, vigor index, hypocotyl lenght, and root length. Seed germination of the early maturing clone started at 202 DAA, while that of the late maturing clone started at 230 DAA, with both clones showing a water content of 63% and a dry mass of 37%. Although the optimum harvest point was identified at the cherry ripening stage, it was found that the late maturing clone showed a more pronounced variation in the maturation process, directly impacting the physiological quality of the seeds. Furthermore, a significant correlation was observed between the maturation stages and the physiological quality of the seeds. For the early maturing clone, the maximum physiological quality was recorded at 244 DAA, corresponding to 80% of fruits at the cherry stage, while for the late maturing clone this point was reached at 326 DAA, with 98% of the fruits ripe.Item Physical and chemical attributes of beans damaged by the coffee berry borer at different levels of infestation(Instituto Agronômico (IAC), 2024-05-09) Silva, Sabrina Alves; Pereira, Rosemary Gualberto Fonseca Alvarenga; Chalfoun, Sara Maria; Teixeira, Alexandre ResendeThe physical and chemical attributes of coffee beans have a direct relation with the sensory quality of the beverage and the commodity’s market value. The coffee berry borer is a pest that causes a worldwide harm, and its infestation compromises bean health and composition. The objective of this study was to analyze the effect of infestation levels on beans physical and chemical parameters. Coffee samples were collected, and the damaged beans present in the samples were classified as to infestation level. The centesimal composition, color, quantification of bioactive compounds, and organic acids were evaluated. The L* and b* parameters of the CIELab color system were found to be significantly different between infestation levels. For chemical parameters, beans with major levels of infestation were found to differ from the others in the content of sugars, lipids, soluble solids, total titratable acidity, electrical conductivity, potassium leaching, succinic acid, and acetic acid. The presence of pests did not affect the bioactive compounds present in the coffee beans.Item Initial vegetative development and early selection of arabica coffee cultivars in a low-altitude region(Instituto Agronômico (IAC), 2023-11-10) Filla, Vinícius Augusto; Coelho, Anderson Prates; Grossi Terceiro, Matheus; Morello, Orlando Ferreira; Lemos, Leandro BorgesThe cultivation of Coffea arabica L. in a low-altitude region is a sustainable alternative for diversifying the income of farmers. On account of the variability of available cultivars, the use of indirect selection through initial vegetative development may be an auxiliary tool for identifying the most suitable genotypes for this environment. This study aimed to identify the dwarf Arabica coffee cultivars with better initial development in a low-altitude region and the morphological traits with greater relevance for the early selection of cultivars with high yield potential. The experiment was installed in the northeast of São Paulo, Brazil. The experimental design used was randomized blocks with four replications. The treatments consisted of 17 dwarf Arabica coffee cultivars. There is variability among dwarf Arabica coffee cultivars for initial vegetative development. The growth and increase in the number of nodes of the plagiotropic branch can be used in early selection to identify cultivars with greater yield potential. The cultivars IAC Obatã 4739, Obatã IAC 1669-20, and Tupi IAC 1669-33 present higher initial vegetative performance in low-altitude environments. The results obtained are useful to breeders and producers for choosing the cultivars best adapted to cultivation in this environment.Item Arbuscular mycorrhizal fungi inoculation for coffee seedling production with commercial and conventional substrates(Instituto Agronômico (IAC), 2023-05-22) Silveira, Adriana Parada Dias; Tristão, Fabrício Sales Massafera; Fernandes, Ana Olívia; Andrade, Sara Adrian Lopez; Cipriano, Mateus Aparecido PereiraCoffee seedlings are commonly produced on substrate composed of a mixture of soil and cattle manure, supplemented with chemical fertilizers. Alternatives to reduce production costs and produce seedlings of greater quality and health include the use of commercial organic substrates, which require less handling. The use of beneficial microorganisms such as arbuscular mycorrhizal fungi (AMF) can be considered a good alternative for production of more vigorous coffee seedlings. The main goal of this study was to evaluate the effect of the inoculation of AMF isolates on coffee seedlings development in a commercial organic substrate (based on coconut fiber) and conventional substrate (mixture composed of soil and cattle manure compost). Ten AMF were tested: Rhizophagus irregularis, Glomus macrocarpum, Claroideoglomus etunicatum, Rhizophagus clarus, Glomus spp., Gigaspora margarita, Acaulospora morrowiae, Acaulospora scrobiculata, Acaulospora spp., and Dentiscutata heterogamma. Plant growth, shoot P content, mycorrhizal colonization, extraradical mycelium length, phosphatase activity, and photosynthetic pigments were evaluated. The effects of mycorrhization depended on both the inoculated fungal species and the substrate for seedling cultivation. Inoculation of G. margarita, Acaulospora spp., and Glomus spp. in the conventional substrate conferred the best growth plant responses, increasing shoot biomass by 160 to 320%. In the commercial substrate, the most efficient AMF were R. clarus, Glomus spp, A. morrowiae and A. scrobiculata, with up to 149% of shoot biomass increase. The commercial organic substrate and the inoculation of some of the AMF isolates were highly beneficial to coffee seedlings development and can replace the use of the conventional substrate. These results open new opportunities for the use of AMF as an inoculant to improve coffee seedling production in commercial organic substrates.Item Multiple-trait model by Bayesian inference applied to environment efficient Coffea arabica with low-nitrogen nutrient(Instituto Agronômico (IAC), 2023-04-14) Silva Júnior, Antônio Carlos da; Moura, Waldênia de Melo; Torres, Lívia Gomes; Santos, Iara Gonçalves dos; Silva, Michele Jorge da; Azevedo, Camila Ferreira; Cruz, Cosme DamiãoIdentifying Coffea arabica cultivars that are more efficient in the use of nitrogen is an important strategy and a necessity in the context of environmental and economic impacts attributed to excessive nitrogen fertilization. Although Coffea arabica breeding data have a multi-trait structure, they are often analyzed under a single trait structure. Thus, the objectives of this study were to use a Bayesian multitrait model, to estimate heritability in the broad sense, and to select arabica coffee cultivars with better genetic potential (desirable agronomic traits) in nitrogen-restricted cultivation. The experiment was carried out in a greenhouse with 20 arabica coffee cultivars grown in a nutrient solution with low-nitrogen content (1.5 mM). The experimental design used was in randomized blocks with three replications. Six agromorphological traits of the arabica coffee breeding program and five nutritional efficiency indices were used. The Markov Chain Monte Carlo algorithm was used to estimate genetic parameters and genetic values. The agromorphological traits were considered highly heritable, with a credibility interval (95% probability): H2 = 0.9538 – 5.89E-01. The Bayesian multitrait model presents an adequate strategy for the genetic improvement of arabica coffee grown in low-nitrogen concentrations. Coffee arabica cultivars Icatu Precoce 3282, Icatu Vermelho IAC 4045, Acaiá Cerrado MG 1474, Tupi IAC 1669-33, Catucaí 785/15, Caturra Vermelho and Obatã IAC 1669/20 demonstrated greater potential for cultivation in low-nitrogen concentration.