Biblioteca do Café
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Item Analysis of the chemical composition and glyphosate residue in Conilon coffee beans(Universidade Federal do Ceará, 2024-05-17) Peixoto, Priscilla Moreira Curtis; Osório, Vanessa Moreira; Colodetti, Tafarel Victor; Parreira, Luciana Alves; Dalvi, Leandro Pin; Tomaz, Marcelo AntonioDu e to the concern with the contamination of food by pesticides, especially coffee, the aim of this study was to analyze the chemical composition and glyphosate residue in Conilon coffee beans with and without defects and harvested at different times after applying the herbicide. The experiment was in a split-plot design, with the presence or absence of defects in the beans comprising the subplots, and the periods of 15, 30, 45 and 60 days after applying the herbicide corresponding to the split plots. We analyzed the volatile compounds, bioactive compounds, and glyphosate residue in the beans. Regardless of the presence or absence of defects, there was an increase in the concentrations of bioactive compounds in the raw Conilon coffee beans as the interval between applying the herbicide and harvesting the beans was increased. The most abundant volatile compounds per percentage area belonged to the pyrazines, furans and phenols. The amount of glyphosate residue found in the beans exceeded the maximum detectable limit by the ELISA method regardless of the time between application and harvest, corresponding to values that are unacceptable to several purchasing countries, and making the samples unsuitable for export and a risk to food safety.Item Analysis of bioactive compounds, organic acids, and genetic parameters of ten amazonian robusta cultivars(Universidade Federal Rural do Semi-Árido, 2024-04-29) Tadeu, Hugo C.; Ximenes, Valdecir F.; Lopes, Maria T. G.; Espindula, Marcelo C.; Alves, Ana P. de C.; Borém, Flávio M.Coffea canephora beans are used for various industrial purposes, among which the use as soluble coffees stands out for producing beverages in blends with Coffea arabica. Due to the increase in demand, EMBRAPA launched ten monoclonal C. canephora cultivars, named Amazonian Robustas, adapted to the growing conditions of the Brazilian Amazon. However, the chemical composition of the beans of these cultivars is still little known. The present study aimed to estimate genetic parameters for the evaluated characteristics and determine the levels of bioactive compounds and organic acids in ten C. canephora cultivars. The experiment was set in Manaus, Amazonas, consisting of plants from the cultivars BRS 1216, BRS 2299, BRS 2314, BRS 2336, BRS 2357, BRS 3137, BRS 3193, BRS3210, BRS 3213, and BRS 3220. The cultivars were characterized according to the profile of bioactive compounds and organic acids. Analysis of variance, mean test, and genetic caracterizadas quanto ao perfil de bioativos e ácidos orgânicos. Foi parameters (genetic, environmental, and phenotypic variance and realizada análise de variância, teste de médias e estimados heritability) were conducted. The heritability of characters was parâmetros genéticos como variância genética, ambiental, fenotípica considered from intermediate 63.76% (trigonelline) to high 88.44% e herdabilidade. A herdabilidade dos caracteres foi considerada de (caffeine). Of the compounds studied, trigonelline contents ranged mediana, 63,76%, para trigonelina, a alta, 88,44%, para cafeína. Dos from 0.54 to 0.78 g.100g-1, chlorogenic acids from 3.77 to 5.31 compostos estudados, os teores de trigonelina variaram de 0,54 a g.100g-1, caffeine from 2.31 to 4.13 g.100g-1, and citric acid from 0.76 to 1.28 g.100g-1. It was observed that there is genetic variability among the cultivars for the compounds studied, and the cultivars can be used in breeding programs for the development of new cultivars.Item Plant stem cell extract from Coffea canephora shows antioxidant, anti-inflammatory, and skin regenerative properties mediated by suppression of nuclear factor-κB(Associação Brasileira de Divulgação Científica, 2023-06-30) Guidoni, M.; Sousa Júnior, A.D. de; Aragão, V.P.M.; Toledo e Silva, M.V.; Barth, T.; Clarindo, W.R.; Endringer, D.C.; Scherer, R.; Fronza, M.Plant cell cultures have become a promising production platform of bioactive compounds for biomedical and cosmetic uses in the last decades. However, the success so far has been limited. The study aimed to evaluate the effectiveness of this unique biotechnology process to obtain a bioactive stem cell extract of Coffea canephora (SCECC) with antioxidant, anti-inflammatory, and regenerative properties. Total phenolic and flavonoid contents were determined in the SCECC by spectrophotometry. The chemical composition of the extracts was characterized by mass spectrometry. Antioxidant activity was evaluated using the colorimetric methods of free radical scavenging 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and the ferric reducing ability of plasma (FRAP). The anti-inflammatory activity was determined in lipopolysaccharide-stimulated RAW 264.7 macrophages through the production of superoxide anion (O2•-), nitric oxide (NO), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and the activity of nuclear factor kappa B (NF-κB). Moreover, the ability of SCECC to stimulate the proliferation and migration of fibroblasts was assessed. Five compounds were tentatively identified, two flavonoids, two phenolic acids, and one sugar. High phenolic content and antioxidant activity were observed in the SCECC. SCECC promoted the proliferation and migration of fibroblasts and suppressed the pro-inflammatory mediators O2•-, NO, TNF-α, and IL-6 in a dose-dependent manner. Moreover, SCECC inhibited the NF-κB transcription factor. Therefore, we obtained evidence that the extract from C. canephora stem cells can be used as a natural agent against skin damage. Hence, it could be of interest in cosmetics for preventing skin aging.Item A design of experiment strategy for quality control of specialty coffee drink based on sensory analysis and statistical tools(Instituto de Tecnologia de Alimentos – ITAL, 2024-12-06) Pinto, Licarion; Lopes Júnior, Hilton; Alves, Enrique Anastácio; Rocha, Rodrigo Barros; Teixeira, Alexsandro Lara; Gois, Jefferson Santos deSensory analysis is crucial for assessing food and beverage quality, but discrepancies may arise in some cases and make the quality evaluation imprecise even with statistical analysis. This issue can be mitigated by sensory analysis based on the coffee drink nuanced characteristics. Therefore, in this study, six Q-Graders, following the Specialty Coffee Association of America's sensory analysis method, evaluated various samples and the data was evaluated statistically. The experiment employed a multilevel categorical design, encompassing six evaluator levels, 44 sample levels, and two types of quality assessment. Grades were assigned to the 44 samples using traditional evaluation (no comments) and guided evaluation (prior sample information). It was possible to identify evaluators whose assessments were unbiased across both guided and traditional evaluations. This work introduced a novel strategy to identify biased evaluators, assess the impact of evaluation types, and perform a more accurate assessment of Coffea canephora Pierre ex-Froehner (coffee) quality and assurance assisted with statistical analysis.Item From anthesis to harvest: evolution of the fructification of Conilon coffee genotypes grown at a transitional altitude(Instituto Agronômico (IAC), 2025-07-07) Colodetti, Tafarel Victor; Tomaz, Marcelo Antonio; Sartori, Lucas; Salles, Rodrigo Amaro de; Souza, Inês Viana de; Senra, João Felipe de Brites; Rodrigues, Wagner NunesBeginning with anthesis, this study aimed to evaluate the growth evolution of fruits of nine genotypes of Conilon coffee with an early cycle of maturation that were cultivated at a transitional altitude. The experiment was conducted in a competition field with Coffea canephora genotypes at a 647-m altitude, following a randomized block design with nine treatments, four replicates, and the genotypes making up the cultivar “Diamante ES8112” (101, 102, 103, 104, 105, 106, 107, 108, and 109). From the anthesis day, evaluations were made every 28 days, ending with the harvest, at which time the characteristics of growth and biomass accumulation of flowers and fruits of each genotype were evaluated. All analyzed characteristics of the flowers and fruits cultivated at a transitional altitude showed differentiation between the nine early-maturing Conilon genotypes. Altitude cultivation increased the duration of fruit development phases, averaging from 252 to 308 days among genotypes, from anthesis to harvest. Genotype 106 stood out for the fewest flowers and fruits per reproductive node and lowest fruit holding rates and dry biomass, whereas genotype 108 stood out for the most flowers and fruits per reproductive node, largest flower diameter, highest fruit holding rate, and greatest dry biomass.Item Maturation cycle and fruit-to-bean conversion ratios in amazon robusta coffee cultivars(Universidade Federal Rural do Semi-Árido, 2024-12-02) Sousa, Andrey L. B. de; Rocha, Rodrigo B.; Tadeu, Hugo C.; Lopes, Maria T. G.; Espindula, Marcelo C.; Silva, Raniel C. da; Ferreira, Fábio M.This study aimed to characterize the outturn index, field outturn index, uniformity of maturity, and maturation cycle of fifteen Coffea canephora genotypes grown in different environments of the Western Amazon. Conducted in Amazonas (Itacoatiara and Manaus) and Rondônia (Porto Velho), the research evaluated the performance of ten cultivars and five promising genotypes from Embrapa. The genotype × environment interaction was significant, indicating different performance of clones across environments. Genotypic coefficients surpassed environmental ones, indicating a genetic influence on outturn indices and uniformity of maturity. The mean outturn index was 24.68%, and the field outturn index was 22.57%, with Itacoatiara having the highest mean values. The overall mean fruit uniformity of maturity was 63.02%, with Porto Velho achieving the highest mean uniformity value (71.78%). The cultivar BRS1216 exhibited the best performance for outturn indices and provided the highest gain from selection across environments, showing wide adaptability for the outturn index and adaptability to environments favorable for field outturn. Cultivars BRS3210 and BRS3220 achieved more than 82% uniformity of maturity across locations, with BRS3210 adapting to favorable environments and BRS3220 adapting to unfavorable ones. Additionally, BRS3220 had a high mean field outturn index, indicating wide adaptability and high phenotypic stability. The evaluated Amazon Robusta clones and cultivars displayed the expected maturation cycles.Item Nutrient content and cutting anatomy can affect the production of Conilon clonal plantlets(Editora UFLA, 2024-09-19) Bazoni, Patricia Alves; Espindula, Marcelo Curitiba; Araújo, Larissa Fatarelli Bento de; Vasconcelos, Jaqueline Martins; Giuriatto Júnior, Jurandyr José Ton; Campanharo, MarcelaCutting is the main vegetative propagation method used to produce Coffea canephora plantlets. In this method, the nutritional quality of the vegetative propagule (stem cuttings) is one of the determining factors for the rooting speed and the final quality of the plantlets. Thus, the objective in this study was to verify possible variations in nutrient content and anatomical characteristics in cuttings collected at different times of the year and their relationship with the production of Coffea canephora clonal plantlets. The study was divided into two phases: 1) Nutritional composition and anatomy of C. canephora cuttings grown at different times; 2) Production of C. canephora seedlings under greenhouse conditions. The treatments consisted of cuttings collection and plantlets production at different times of the year: January, May and September 2017. We observed that there is seasonal variation for the content of N, P, K and Mg; and anatomical changes in xylem, phloem and vascular cylinder thickness in cuttings harvested at different times of the year. We conclude that although nutritional and anatomical aspects of the vegetative propagule may result in different vegetative growth rates of C. canephora clonal plantlets, this result is more dependent on the management of the nursery environment conditions, especially temperature and relative air humidity.Item Magnus grano: Maximum quality in bean size of conilon coffee(Crop Breeding and Applied Biotechnology, 2024-08-25) Sousa, Priscila Gonçalves Figueiredo de; Vieira, Henrique Duarte; Partelli, Fábio Luiz; Santos, Eileen Azevedo; Viana, Alexandre Pio; Souza, Rosenilda de; Oliosi, Gleison; Correia, Laísa Zanelato; Acha, Amanda JustinoCultivar Magnus grano is made up of five genotypes that were selected by coffee growers in the state of Espírito Santo, Brazil. The cultivar shows good adaptation to growing conditions, high yield potential, and the largest beans among the studied genotypes.Item Can season and intercropping Conilon coffee favor predatory ant species?(Universidade Federal de Lavras, 2024-06-18) Martins, David dos Santos; Fornazier, Maurício José; Abonizio-Santos, Marlina Ribeiro; Guarçoni, Rogério Carvalho; Teixeira, Alex Fabian Rabelo; Magalhães, Fabrício Severo; Souza-Campana, Débora Rodrigues; Maria Santina de Castro, Maria Santina de CastroConilon coffee (Coffea canephora Pierre ex A. Froehner) is an important crop in Brazil, and this culture is widely grown in Espírito Santo State. Intercropping this species has been adopted for better use, profitability, and sustainability of the cultivated area. Intercropping provides higher shading and may favor edaphic fauna that plays an important ecological function in the soil. Ants are part of the edaphic meso - and macrofauna, considered ecosystem engineers and actively participate in pest predation. The goal of the study was to evaluate predatory ant richness in Conilon coffee in monoculture and intercropped with teak or Australian cedar. Furthermore, the influence of these crops on the richness of predator ant communities in the dry (winter) and rainy (summer) seasons was analyzed. The study was conducted in the municipality of Sooretama, northern Espírito Santo State (Brazil) using pitfall soil traps. Ant communities were analyzed with their distribution in trophic guilds. The richness of predatory ants (predatory and generalist omnivore predatory ant species) was studied in Conilon coffee in monoculture and intercropped with teak (Tectona grandis L.f.) and with Australian cedar (Toona ciliata M. Roemer), in the dry (winter) and wet (summer) seasons. A total of 49 ant species were collected, 29 predatory and 15 generalist omnivore predatory ant species were identified, and 16 ant species were recorded for the first time in Espírito Santo State. Conilon coffee cropping systems did not affect the richness of predatory or generalist omnivore predatory ant species, however, summer favored the occurrence of total predatory ants (generalist omnivore + predators); intercropping Conilon coffee did not favor predatory ants. The presence of predatory species is important for natural pest control, consequently improving crop sustainability. However, new research must be done to evaluate other Conilon coffee intercropping for a longer period.Item Water requirement satisfaction index for coffee (Coffea canephora) crops in Western Amazon(Departamento de Engenharia Agrícola - UFCG, 2024-07-30) José, Jefferson V.; Pereira, Lívia B.; Souza, Moisés D.; Leite, Kelly N.; Santos, Otávio N. A.; Santos, Lucas C.Water balance is a tool that has various applications in agriculture, including assessing whether an environment is suitable for growing a specific crop by providing information on how water resources function in the system. This information is essential for determining whether the available water satisfies the crop’s demand. Thus, the objective of this work was to calculate the water balance of Typic Hapludults for growing coffee (Coffea canephora) crops in Cruzeiro do Sul, Acre, Brazil, using a 36-year historical series to assess the potential and limitation of the crop according to the water availability. The results showed that coffee crops are, in general, suitable for growing in the study region. Water deficit and water surplus periods are well-defined, from April to August and September to March, respectively. Flowering was the only stage of coffee crops that coincides with the water deficit period in the region; thus, irrigation is necessary to prevent compromising this stage and fruit development. The Water Requirement Satisfaction Index (WRSI), calculated on a monthly basis, indicates a low climate risk for coffee crops in the region. However, when calculated on a daily basis, the WRSI shows a medium to high climate risk for coffee crops in several periods. The WRSI proved to be an adequate tool for assisting in decision-making regarding the adoption of irrigation.