Biblioteca do Café
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Item Analysis of bioactive compounds, organic acids, and genetic parameters of ten amazonian robusta cultivars(Universidade Federal Rural do Semi-Árido, 2024-04-29) Tadeu, Hugo C.; Ximenes, Valdecir F.; Lopes, Maria T. G.; Espindula, Marcelo C.; Alves, Ana P. de C.; Borém, Flávio M.Coffea canephora beans are used for various industrial purposes, among which the use as soluble coffees stands out for producing beverages in blends with Coffea arabica. Due to the increase in demand, EMBRAPA launched ten monoclonal C. canephora cultivars, named Amazonian Robustas, adapted to the growing conditions of the Brazilian Amazon. However, the chemical composition of the beans of these cultivars is still little known. The present study aimed to estimate genetic parameters for the evaluated characteristics and determine the levels of bioactive compounds and organic acids in ten C. canephora cultivars. The experiment was set in Manaus, Amazonas, consisting of plants from the cultivars BRS 1216, BRS 2299, BRS 2314, BRS 2336, BRS 2357, BRS 3137, BRS 3193, BRS3210, BRS 3213, and BRS 3220. The cultivars were characterized according to the profile of bioactive compounds and organic acids. Analysis of variance, mean test, and genetic caracterizadas quanto ao perfil de bioativos e ácidos orgânicos. Foi parameters (genetic, environmental, and phenotypic variance and realizada análise de variância, teste de médias e estimados heritability) were conducted. The heritability of characters was parâmetros genéticos como variância genética, ambiental, fenotípica considered from intermediate 63.76% (trigonelline) to high 88.44% e herdabilidade. A herdabilidade dos caracteres foi considerada de (caffeine). Of the compounds studied, trigonelline contents ranged mediana, 63,76%, para trigonelina, a alta, 88,44%, para cafeína. Dos from 0.54 to 0.78 g.100g-1, chlorogenic acids from 3.77 to 5.31 compostos estudados, os teores de trigonelina variaram de 0,54 a g.100g-1, caffeine from 2.31 to 4.13 g.100g-1, and citric acid from 0.76 to 1.28 g.100g-1. It was observed that there is genetic variability among the cultivars for the compounds studied, and the cultivars can be used in breeding programs for the development of new cultivars.Item Effect of Microwave Use on Kawa Daun Production: Production, Composition, and Antioxidant Properties(Editora UFLA, 2025-06-03) Arief, Rastra Gemi Nastiti; Malrianti, Yefsi; Kasim, Anwar; Neswati; SujatmikoKawa daun tea is a drink made of dried coffee leaves from Western Sumatra, offering refreshing benefits for the body. The use of a microwave in the drying process proves to be an effective method due to its significantly high efficiency. Therefore, this study aimed to investigate the impact of varying drying time using microwave on the characteristics of coffee leaves, kawa daun tea, and drinking water, as well as to determine effect of microwave treatment. This study uses a complete random design with ANOVA (Analysis of Variance) and followed by a DNMRT (Duncan’s News Multiple Range Test). The results showed that the difference in drying times significantly affected properties of coffee tea leaves, specifically on the total water-soluble substance test. However, there was no substantial impact on the yield, water level, polyphenols level, caffeine level, and antioxidant activity. The length was also affected by polyphenols level, total soluble solidity, and water solubility antioxidant activity, but did not impact caffeine content test. The maximum time for optimal drying coffee leaves was two minutes with a yield of 35.99% hydration, 4.33% water level, 48.56 mgGAE/g total polyphenols level, 0.56% caffeine level, 63.43% oxidative activity, and 4.61% total water-soluble material. In the chemical analysis of drinking water, the results obtained were total polyphenol content of 28.1 mg GAE/g, caffeine content of 0.45%, total dissolved solids of 1.87%, and antioxidant activity of 88.79%. Thus, it is also concluded that there is no negative effect of drying coffee leaves with a microwave.Item Antioxidant Activity and Phytochemicals of Freeze-dried and Spray-dried Soluble Coffee Brews(Editora UFLA, 2025-05-19) Susilawati, I Dewa Ayu; Muzeka, FrediThis study analyzed the antioxidant activity and the concentration of several phytochemicals i.e. polyphenols, caffeine, glutathione (GSH), and protein, of freeze-dried and spray-dried soluble coffee brews. Six types of coffee samples were analyzed i.e., 1) Spray-Dried Robusta instant coffee (SDR), 2) Freeze-Dried Robusta instant coffee (FDR), 3) Regular Robusta coffee powder (RR), 4) Spray-Dried Arabica instant coffee (SDA), 5) Freeze-Dried Arabica instant coffee (FDA), and 6) Regular Arabica coffee powder (RA). Antioxidant activity was determined using the 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) radical scavenging assay. Polyphenols, caffeine, glutathione, and protein were measured spectrophotometrically. Results showed that freeze-dried instant coffee (both robusta and arabica) in a concentration of 500 ppm demonstrated DPPH radical scavenger activity >80% and significantly higher (p<0.05) compared to spray-dried soluble coffee and regular coffee brews. This level was maintained in concentrations of 750 ppm, 1000 ppm, and 1500 ppm. The higher antioxidant activity of freeze-dried coffee brew was in line with the higher concentration of polyphenols, caffeine, GSH, and protein. In conclusion, freeze-dried instant coffee demonstrated more sensitive antioxidant activity, probably due to its more abundant polyphenols, caffeine, GSH, and protein.Item Coffee and blood pressure(Universidade Federal de Lavras, 2024-06-17) Nurvita, Silvia; Rizkaprilisa, WindyCoffee has a characteristic taste, aroma and color that attracts its fans. Coffee is one of the drinks most consumed after mineral water. Annual coffee consumption is estimated at around 500 billion cups/year. Coffee contains a lot of caffeine and other chemical compounds. Coffee having a positive impact on health, also has a negative impact on health. The negative impact of coffee on health is that coffee affects blood pressure (BP) and the risk of hypertension. This research aims to determine the effect of coffee on blood pressure according to existing research. This research method uses the Literature Review (LR) research type. The article inclusion criteria used for this research were articles published in 2020-2023 with the keywords coffee, blood pressure, the relationship between coffee and blood pressure, the effect of coffee and blood pressure. This research found three articles that met the inclusion criteria for this research. The results of this research explain that three articles, there are two articles which prove that coffee consumption has no effect on blood pressure, but there is one article which shows that the duration of coffee consumption has an effect on systolic blood pressure. The conclusion of this literature study from these three articles can be concluded that on average coffee consumption has no effect on blood pressure. Coffee that is good for health is pure coffee that is brewed without a sugar.Item Roasting variations and brewing methods in the preparation of clear Coffee Beverages from Gayo Arabica Coffee(Universidade Federal de Lavras, 2024-07-29) Sulaiman, Ismail; Marsyanda; Erika, CutCoffee drinks are trendy drinks around the world, and they usually contain caffeine. However, this research focuses on creating a coffee drink that does not contain caffeine so that those who cannot consume it can enjoy it. The resulting coffee drink is a colorless coffee drink. The coffee production process initially produces a black-colored drink, but it can turn it into a clear-colored drink through distillation and chemical methods. This research aims to study the process of making clear coffee by varying roasting and brewing levels with various variations to obtain clear-colored coffee and low caffeine levels. Preparation of clear coffee drinks is done by brewing espresso and Tobruk, which is then analyzed for pH, caffeine, and color tests on the results of the clear coffee drink. Based on the ranking test on each parameter tested on clear coffee drinks, the best treatment is using the distillation method, espresso brewing, and medium roasting level. The analysis results of this selected formulation produced a transparent beverage color (clear), pH 3.5, and caffeine levels of 4.639 mg/mL.Item Green coffee extract attenuates Parkinson’s-related behaviors in animal models(Academia Brasileira de Ciências, 2021-11-01) Molska, Graziella R.; Paula-Freire, Lyvia Izaura G.; Sakalem, Marna E.; Köhn, Daniele O.; Negri, Giuseppina; Carlini, Elisaldo A.; Mendes, Fúlvio R.Epidemiological studies have shown an inverse association between coffee consumption and the development of Parkinson’s disease (PD). The effects of the oral treatment with green (non-roasted) coffee extracts (CE, 100 or 400 mg/kg) and caffeine (31.2 mg/kg) were evaluated on catalepsy induced by haloperidol in mice, and unilateral 6-OHDA lesion of medial forebrain bundle (MFB) or striatum in rats. Also, the in vitro antioxidant activity and the monoamine levels in the striatum were investigated. CE presented a mild antioxidant activity in vitro and its administration decreased the catalepsy index. CE at the dose of 400 mg/kg induced ipsilateral rotations 14 days after lesion; however, chronic 30-day CE and caffeine treatments did not interfere with the animals’ rotation after apomorphine or methamphetamine challenges in animals with MFB lesion, nor on monoamines levels. Furthermore, CE and caffeine were effective in inhibiting the asymmetry between ipsilateral and contralateral rotations induced by methamphetamine and apomorphine in animals with lesion in the striatum but did not avoid the monoamines depletion. These results indicate that CE components indirectly modulate dopaminergic transmission, suggesting a pro-dopaminergic action of CE, and further investigation must be conducted to elucidate the mechanisms of action and the possible neuroprotective role in PD.Item Validação do questionário de expectativa à cafeína para a população brasileira e associação aos polimorfismos dos genes CYP1A2 e ADORA2A(Universidade de Brasília, 2022-06-29) Mendes, Guilherme Falcão; Zandonadi, Renata Puppin; Reis, Caio Eduardo GonçalvesA expectativa de efeitos da cafeína pode ser registrada em questionários validados para observar padrões favoráveis, ou não, ao uso de fontes cafeína. Esta individualidade biológica está relacionada com os polimorfismos genéticos podem interferir no modo como os indivíduos metabolizam a cafeína, gene CYP1A2 (rs 762551), genótipo AA, fenótipo rápidos metabolizadores, e a sua ação nos receptores de adenosina ADORA2A (rs 5751876), genótipo TT, fenótipo maior sensibilidade. Portanto, o presente estudo teve como objetivo avaliar a capacidade do CaffEQ-BR na diferenciação dos polimorfismos dos genes CYP1A2 e ADORA2A, conforme as expectativas de efeito da cafeína medidas pelo instrumento. O estudo foi composto por três etapas: (i) processo de tradução com dois tradutores bilingues, validação semântica por meio de técnica de juízes (n = 20), análise de reprodutibilidade e consistência interna com amostra de conveniência (n = 50) e validação externa por meio da análise fatorial confirmatória com amostra composta por 4.202 participantes de todas as Unidades da Federação; (ii) desenvolvimento e validação da versão curta do instrumento (B-CaffEQ-BR); (iii) aplicação do CaffEQ-BR (versão completa e curta) a uma amostra (n = 71) de atletas brasileiros treinados em desenvolvimento, consumidores habituais de cafeína com determinação prévia para os polimorfismos dos genes CYP1A2 e ADORA2A. O questionário se mostrou adequado quanto à confiabilidade, clareza e compreensão. A reprodutibilidade e a validação foram confirmadas pelo alfa de Cronbach (α) de 0,948, e foi observado um coeficiente de correlação intraclasse de 0,976. Os sete fatores apresentaram um bom ajuste para a raiz do erro quadrático médio de aproximação – RMSEA = 0,0332 (IC 95%: 0,0290–0,0375). Após a validação externa, o CaffEQ-BR passou por modelagem estatística com vistas a reduzir o número de itens, com três itens por fator, mantendo os sete fatores (CaffEQ-BR versão curta com 21 itens) com reprodutibilidade interobservador e a consistência interna tão satisfatória quanto o CaffEQ-BR (α de Cronbach ≥ 0,729) e reprodutibilidade global (ICC ≥ 0,915) para todo o questionário e seus sete fatores. As versões completa e curta foram aplicados em indivíduos (n = 71) com genotipagem prévia para os polimorfismos dos genes CYP1A2 e ADORA2A. A frequência observada dos genótipos AA para o gene CYP1A2 foi de 47,9% (n=34) e portadores do alelo C (AC e CC) foi de 52,1% (n=37). Para o gene ADORA2A foi observado 22,7% (n=15) como portadores do genótipo TT e 77,3% vii (n=51) portadores C (TC e CC). Com exceção ao fator “ansiedade/efeitos físicos negativos”, os demais escores do CaffEQ-BR (completo e curto), obtiveram ICC > 0,75. Indivíduos que pontuaram > 4 na escala Likert (“um pouco provável”) no fator ansiedade/efeitos negativos no B-CaffEQ-BR apresentaram capacidade discriminatória para o genótipo TT para ADORA2A (p = 0,01) de acordo com a curva ROC, mas com representatividade muito baixa (n = 2). Portanto, na presente amostra estudada, o CaffEQ-BR não foi capaz de diferenciar, por meio da expressão dos fenótipos de rápida metabolização hepática e maior sensibilidade na ação da cafeína nos receptores de adenosina, associado aos genótipos para os genes CYP1A2 e ADORA2A em amostra de conveniência de atletas brasileiros treinados em desenvolvimento. Sugere-se que pesquisas futuras utilizem amostras mais amplas, com grupo controle composto por indivíduos com consumo baixo ou irregular consumo de cafeína, calibrando o questionário com maior foco em aspectos da ansiedade e efeitos negativos na busca em diferenciar o genótipo TT para ADORA2A.Item Extraction of caffeine, chlorogenic acids and lipids from green coffee beans using supercritical carbon dioxide and co-solvents(Brazilian Society of Chemical Engineering, 2008-09-02) Azevedo, A. B. A.; Mazzafera, Paulo; Mohamed, R. S.; Melo, S. A. B. Vieira de; Kieckbusch, T. G.The paper reports on experimental data on the extraction of caffeine, coffee oil and chlorogenic acids from green coffee beans using pure supercritical CO2 and supercritical CO2 modified with ethanol (5% w/w) and isopropyl alcohol (5% w/w) at 50 and 60ºC and 15.2 24.8 e 35.2 MPa. In this study extraction kinetics were obtained for all assays i.e. samples were collected at several time intervals for each solvent and mixed solvent. When pure CO2 and CO2-ethanol mixed solvent were used, an increase in pressure resulted in an increase in the amount of oil extracted. When CO2 was modified with isopropyl alcohol, the amount of coffee oil extracted also increased with pressure. Caffeine extraction initially increased and subsequently decreased with pressure. Chlorogenic acids were only extracted when isopropyl alcohol was used as a co-solvent. An increase in extraction temperature resulted in a decrease of caffeine and oil extraction (retrograde condensation) when only CO2 was used as solvent. With the use of co-solvent this retrograde behavior was no longer observed and the increase in temperature resulted in the increase in the extracted amounts of caffeine, coffee oil and chlorogenic acids.Item Bioactive compounds, antioxidant activity, physical and sensory characteristics of Mırra coffee(Sociedade Brasileira de Ciência e Tecnologia de Alimentos, 2022-03-18) Yalçinkaya, Cihan; Abdalla, Hassan Sarbaz; Bakkalbasi, EmreMırra is a coffee beverage widely consumed in Syria and Turkey, and often produced from roasted and ground coffee beans. Recently, it is prepared from classical instant coffee. In this study, some physicochemical, bioactive and sensory properties of Mırra samples were determined. The average viscosity, °Brix, HMF, total phenolic content, DPPH and ABTS values were 1.36 cP, 3.70, 71.60 mg/L, 3431.55 mg GA eq./L, 6.24 mmol Trol. eq./mL and 35.23 mmol Trol. eq./mL for Mırra samples made by traditional process, and 4.85 cP, 16.36, 303.3 mg/L, 11276.47 mg GA eq./L, 23.89 mmol Trol. eq./mL and 89.70 mmol Trol. eq./mL for Mırra samples made with classic instant coffee, respectively. All Mırra samples also contained high levels of caffeine (1416.93 - 4347.46 mg/L). Chlorogenic acid, 4-O-caffeoylquinic acid and trans-5-O-caffeoylquinic acid were identified in Mırra samples. Total chlorogenic acid contents of Mırra samples were ranged from 1097.85 to 5283.21 mg/L. In all sensory parameters, Mırras with °Brix value over 5.75 had high scores. Results show that Mırra has high antioxidant activity. However, Mırra consumption may have negative health effects for risk groups due to the high caffeine content.Item The inclusion of coffee in commercial layer diets(Fundação APINCO de Ciência e Tecnologia Avícolas, 2013) Mendes, L. R.; Silva, R. B.; Bueno, C. F. D.; Couto, F. A. P.; Dias, A. N.; Fernandes, V.; Faria Filho, D. E.This experiment aimed at evaluating the effect of the dietary inclusion of caffeinated and decaffeinated coffee on the performance and internal and external egg quality of commercial layers. One hundred and twenty 25-week-old Hy-line Brown layers, with 1575 ± 91 average body weight, were distributed according to a completely randomized experimental design with three treatments (control, 1.2% caffeinated coffee, or 1.2% decaffeinated coffee) of five replicates of eight birds each. The inclusion of 1.2% caffeinated coffee was calculated to supply 6mg caffeine per kg body weight, which is considered a moderate dose. The applied treatments did not influence (p>0.05) feed intake, egg production, egg weight, egg mass, feed conversion ratio, Haugh units, yolk color or albumen and yolk percentages. The eggs of hens fed 1.2% caffeinated coffee presented lower (p<0.05) eggshell thickness and egg specific density. The eggs of layers fed 1.2% caffeinated coffee tended (p=0.0637) to present lower eggshell percentage. It was concluded that feeding caffeinated coffee to commercial layers does not affect their performance or internal egg quality; however, eggshell quality is impaired.
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