Antioxidant Activity and Phytochemicals of Freeze-dried and Spray-dried Soluble Coffee Brews

Nenhuma Miniatura Disponível

Data

2025-05-19

Título da Revista

ISSN da Revista

Título de Volume

Editor

Editora UFLA

Resumo

This study analyzed the antioxidant activity and the concentration of several phytochemicals i.e. polyphenols, caffeine, glutathione (GSH), and protein, of freeze-dried and spray-dried soluble coffee brews. Six types of coffee samples were analyzed i.e., 1) Spray-Dried Robusta instant coffee (SDR), 2) Freeze-Dried Robusta instant coffee (FDR), 3) Regular Robusta coffee powder (RR), 4) Spray-Dried Arabica instant coffee (SDA), 5) Freeze-Dried Arabica instant coffee (FDA), and 6) Regular Arabica coffee powder (RA). Antioxidant activity was determined using the 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) radical scavenging assay. Polyphenols, caffeine, glutathione, and protein were measured spectrophotometrically. Results showed that freeze-dried instant coffee (both robusta and arabica) in a concentration of 500 ppm demonstrated DPPH radical scavenger activity >80% and significantly higher (p<0.05) compared to spray-dried soluble coffee and regular coffee brews. This level was maintained in concentrations of 750 ppm, 1000 ppm, and 1500 ppm. The higher antioxidant activity of freeze-dried coffee brew was in line with the higher concentration of polyphenols, caffeine, GSH, and protein. In conclusion, freeze-dried instant coffee demonstrated more sensitive antioxidant activity, probably due to its more abundant polyphenols, caffeine, GSH, and protein.

Descrição

Palavras-chave

Caffeine, DPPH, Glutathione, Polyphenols, Protein

Citação

SUSILAWATI, I Dewa Ayu; MUZEKA, Fredi. Antioxidant activity and phytochemicals of freeze-dried and spray-dried soluble coffee brews. Coffee Science, Lavras, v. 20, p. 01-05, 2025.

Avaliação

Revisão

Suplementado Por

Referenciado Por