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Item Large-scale gene expression analysis reveals the role of primary metabolism regulation in resistance to Brazilian Pseudomonas coronafaciens pv. garcae in coffee(Instituto Agronômico (IAC), 2024-12-20) Schenk, Juliana Camargo Martinati; Rodrigues, Lucas Mateus Rivero; Arruda, Natália; Guimarães, Paula de Souza; Diniz, Leandro Cardamone; Rezende, Antonio Mauro; Destéfano, Suzete Aparecida Lanza; Padilha, Lilian; Maluf, Mirian Perez; Guerreiro Filho, OliveiroThis study investigated the response of arabica coffee plants to the pathogen Pseudomonas coronafaciens pv. garcae using RNA-seq technology. Susceptible and resistant coffee plants were inoculated with the bacteria, and leaf samples were collected at different time points for RNA sequencing. Seven genes related to different defense pathways were chosen for expression quantification in time-course experiments using infected leaves from resistant and susceptible plants, as well as non-infiltrated and water-infiltrated leaves as controls. The results obtained revealed that response mechanisms differ between genotypes and provide insights into the genetic basis of early defense in coffee plants against P. coronafaciens pv. garcae, offering potential strategies for genetic breeding.Item Rock powder application combined with bacterial inoculation enhances the early growth of coffee plants(Editora UFLA, 2025-03-14) Corrêa, Jessiane dos Santos; Araújo, Dayane Aparecida de Oliveira; Rodrigues, Allieksiei Castelar Perim Souza; Brito, Orlando Gonçalves; Gusmão Júnior, Genilson Rodrigues; Rodrigues, Tatiana Tozzi Martins SouzaThe use of rock powder combined with nutrient-solubilizing and growth-promoting bacteria represents a promising alternative for nutrient supply to the soil and improved crop development. This study aimed to evaluate the development of coffee seedlings treated with rock dust and inoculated with Bacillus species. Six types of rock powder (slate, black slate, gneiss, kamafugite, pyroxenite, and a mixture of mica schist and granite) were added to standard fertilizer in the presence or absence of a Bacillus mixture (Bacillus subtilis and Bacillus amyloliquefaciens) during coffee cultivation. We measured plant height, stem diameter, leaf number, shoot and root dry biomass, and nutrient levels in the soil and coffee leaves after. In the kamafugite treatment, adding a bacterial mixture increased plant height. When the bacterial application was considered alone, it resulted in an 85% increase in the plants’ total dry biomass. Rock powder and bacteria showed a substantial interaction effect, particularly for nutrients. Gneiss and kamafugite treatments increased phosphorus availability in the soil, whereas bacterial presence boosted calcium and magnesium content. The combination of nutrient-solubilizing and growth-promoting bacteria and rock powder improved soil chemical properties and the early development of coffee plants.Item Sample size estimation of fruit maturation for Arabica’s coffee(Instituto Agronômico (IAC), 2025-01-31) Botega, Gustavo Pucci; Abrahão, Juliana Costa de Rezende; Botelho, Thiago Tavares; Botelho, Cesar Elias; Salvador, Guilherme Soares; Gonçalves, Flávia Maria AvelarThis study aimed at establishing the ideal sample size for evaluating the maturation cycle in Coffea arabica, and investigating the errors associated with different sample sizes, in addition to verifying the possibility of using the clustering method to separate genotypes according to the maturation stage. Two experiments were analyzed: one with F2:3 progenies using visual maturation assessment through fruit counting, and another with cultivars using image processing for maturation assessment. To determine the ideal sample size for this trait, we used the estimation of the errors associated with maturation, using the bootstrap technique. Subsequently, the K-means algorithm was tested as an alternative for clustering genotypes into maturation classes. The application of the bootstrap technique in order to estimate the error associated with maturation revealed that the adoption of a 450-mL sample size resulted in an associated error of approximately 5%, indicating that it is an adequate size for character assessment. The implementation of K-means as a clustering tool offers a promising perspective for Arabica coffee plant breeding programs. A more comprehensive analysis, which not only assesses the proportion of ripe fruits, but also considers the distribution of different maturation stages, provides a more accurate understanding of the maturation process. This allows a more precise identification of genotypes with the most suitable performance for different growing conditions, as well as enabling adjustments in harvest management and post-harvest processing, optimizing coffee quality.Item Contribution of mycorrhiza and phosphate-solubilizing fungi in coffee seedling growth in four soils with different fertility conditions(Instituto Agronômico (IAC), 2025-01-17) González-Osorio, Hernan; Sadeghian, Siavosh; Mira, Beatriz EugeniaPhosphorus (P) is a limiting nutrient for coffee seedlings. To meet this requirement, coffee growers usually apply di-ammonium phosphate (DAP), which is highly sensitive to local price fluctuations. The use of arbuscular mycorrhizal fungi (AMF) and phosphate-solubilizing fungi (PSF) has been recommended, but the results derived from their application are inconsistent and uncertain, depending on soil fertility status. The response of AMF and PSF application in the plant growth of coffee seedlings during the nursery stage was evaluated in four non-sterile soils, which represent the most abundant type of soils in the coffee regions of Colombia: Eutropept, Dystropept, Fulvudand, and Ultisol. The treatments included a commercial AMF inoculum, PSF-Phlebia subserialis-CH4, and a consortium of AMF+PSF, at four P levels (0, 0.5, 1, and 2 g.plant-1 P2O5) using DAP fertilizer. Shoot dry weight (SDW) was affected by P applications in Fulvudand. In the other soils, a P-response occurred with 0.5 g.plant-1 , whereas 1 and 2 g.plant-1 of P2O5 caused a significant reduction in SDW. AMF increased SDW by 40% in Eutropept. The negative effect of higher P amounts was mitigated (> 70%) through PSF and AMF+PSF in Ultisol and Eutropept, respectively. In plants in which microorganisms increased SDW, the P concentration was 0.17 to 0.22%. The plant growth promotion of coffee seedlings during the nursery stage with AMF and/or PSF applications varied according to soil type and P amounts applied.Item Altitude on the physicochemical characteristics of coffee beverage from beans produced in Taquaritinga do Norte, in the state of Pernambuco, Brazil(Empresa Brasileira de Pesquisa Agropecuária - Embrapa, 2025-06-13) Santos, Wallysson Wagner Vilela; Silva, Gustavo Henrique Daniel Santos; Silva, Elisandra Rabêlo da; Silva, Mateus Bezerra de Lima e; Oliveira, Rodrigo Lira de; Silva, Suzana Pedroza daThe objective of this work was to determine the physicochemical characteristics of coffee beverages prepared with Coffea arabica beans produced at different altitudes in the municipality of Taquaritinga do Norte, in the state of Pernambuco, Brazil. Eight coffee bean samples, obtained from five different producers, were analyzed in a completely randomized design, with three replicates. The evaluated variables were: total acidity, pH, electrical conductivity, total dissolved solids, extraction percentage, reducing sugars, and caffeine content. The data were subjected to the analysis of variance, Tukey’s mean test, and agglomerative hierarchical clustering. The results showed total acidity from 0.39 to 3.05 mL-1 NaOH, pH from 5.30 to 4.80, electrical conductivity from 2.79 to 3.24 µS cm-1, total dissolved solids from 2.00 to 2.56 °Brix, extraction percentage from 18.14 to 27.24%, reducing sugars from 0.28 to 0.40%, and caffeine content from 0.50 to 2.18%. Through agglomerative hierarchical clustering, two groups of samples were discriminated based on the evaluated physicochemical parameters. Altitude is a common aspect of the samples of a same group, defining different characteristics of coffee beverages in Taquaritinga do Norte.Item Physiological and nutritional parameters of drought resistance in coffee seedlings genotypes(Editora da Universidade Estadual de Maringá - EDUEM, 2025-06-02) Andreazi, Elder; Sousa, Bruno Teixeira de; Oliveira, Halley Caixeta; Fonseca, Inês Cristina de Batista; Mariucci Junior, Valdir; Shigueoka, Luciana Harumi; Sera, Gustavo HiroshiDrought is an environmental condition that compromises the development of coffee plants. New coffee genotypes that are resistant to drought must be selected quickly and practically. The objectives of this study were to evaluate the resistance of five genotypes of Coffea arabica, including three new genotypes with introgression of genes from Coffea racemosa (H0113-40-26-1, H0113-40-26-19, and H0113-40-26-10), to water restriction and relate the intensity of plant wilting with physiological responses and nutrient accumulation. The experiment was conducted using 45 coffee seedlings obtained from seeds with six pairs of leaves cultivated in tubes. Some seedlings were subjected to two water restriction periods, whereas the remainder were kept under irrigation. The photosynthesis rate, transpiration rate, and wilting intensity were evaluated after each restriction period. Nutrient content was also evaluated after two periods of water restriction. The evaluation of wilting intensity corroborated the physiological parameters. There was a reduction in photosynthesis and transpiration rates under water restriction and nutrient accumulation in coffee seedlings H0113-40-26-1, H0113-40-26-19, and H0113-40-26-10 increased under these conditions. C. arabica genotypes carrying the genes of C. racemosa presented good drought resistance, with H0113-40-26-10 being the most resistant and showing the lowest wilt intensity.Item Identification of morphoagronomic traits correlated with the N use efficiency in coffee(Editora UFLA, 2025-04-29) Moura, Waldenia de Melo; Ribeiro, Poliane Marcele; Soares, Luciana Gomes; Silva Júnior, Antônio Carlos da; Ferreira, Tatiane Cravo; Gravina, Geraldo de Amaral; Martinez, Hermínia Emília PrietoThe study of nutritional efficiency is an expensive process, as it requires extensive planting areas, several years of evaluation and the destruction of plants. To mitigate these difficulties, a strategy would be to identify easily measurable traits associated with nutritional efficiency in growing a nutritional solution. Thus, the objective of this study was identify morphoagronomic traits correlated with the N-efficiency indices in to assist in selecting coffee genotypes for environments with N restriction. Twenty arabica coffee genotypes were grown in a nutrient solution with a low concentration of nitrogen (1.0 mmol L-1). The experiment was conducted in a randomized block design with three replications. There was variability among the coffee genotypes for all the traits evaluated. Most of the traits evaluated showed greater genetic than environmental influence on phenotypic expression. Heritability (H2) was greater than 70% for most of the traits evaluated, with an emphasis on plant height and internode length, which also had the highest relative variation indices (RVIs). The associations between morphoagronomic traits and nutritional efficiency indices revealed greater contributions of genotypic correlation than of environmental correlation. Among the traits associated with nutritional efficiency indices, stem diameter has the potential for use in breeding programs for the selection of cultivars that present greater nitrogen efficiency in environments with nitrogen restriction.Item New orthotropic stems induction in arabica coffee by pruning and biostimulant application(Editora UFLA, 2024-11-11) Honda Filho, Cássio Pereira; Coelho, Larissa Sousa; Andrade, Otavio Vitor Souza; Godinho, Emmanuel ZulloPruning are techniques used to renew a coffee tree and promote the growth of a more vigorous plant. Allied to that, the use of biostimulants can help the boost growth and development of the new produced stems. The objective of the present work was to evaluate whether the presence of plagiotropic branches in coffee trees pruned by low pruning and the application of biostimulant would influence the production, growth and vigor of new orthotopic branches in Coffea arabica. The experiment consisted of 10 treatments, arranged in a randomized block design with a 2x5 factorial: two pruning methods (low pruning with and without plagiotropic branches) and five doses of Stimulate® (0, 100, 200, 400 and 800 mL). The traits evaluated were vigor of the orthotropic branches produced vigor, orthotropic branches length, number of orthotropic branches orthotropic and orthotropic branches diameter. The use of low pruning with remaining plagiotropic stems combined with the use of biostimulants at a dose of 400 mL induces the production of vigorous orthotropic stems in Arabica coffee trees.Item Sensory evaluation and color of Coffea arabica coffee from the Central Mexican Highlands(Editora UFLA, 2024-11-12) Flores, Omar Roberto Vargas; Arriaga, Martín Rubí; Berasain, María Dolores Mariezcurrena; Elías, Guillermo Asdrúb al Vargas; Dávila, José Francisco RamírezCoffee is cultivate d worldwide at an average altitude of 1,500 m worldwide, but in Mexico is typically cultivated around 1,200 m. However, in Temas caltepec, Mexico State, coffee is grownat 2,100 m, resulting in a slower development process that yields a “differentiated quality.” This quality has been nationally recognized atthe “Cup of Excellence” competition, where coffees scoring above ninety points out of a hundred have received the “presidential award.” Thisrecognition has enabled these coffees to be sold as “specialty” coffeeat auction to Europe and Asia, fetching higher prices per pound and thus improving economic returns. In this study, wescientifically validate these claims by evaluating sensory quality in the cup and color ofthe bean. , for which two toasting sta rt temperatures were used. The color in roasted coffee is derived from caramelization due to the high temperatures to which it is subject ed during this process. This characteristic has an intrinsic influence for the taster even though it is not objectively measured during the cupping process. For this reason, it was decided to carry out the measurement in this research. In 2022, Arabica coffee beans of the Caturra variety were harvested from Temascaltepec using a natural process. The coffee was roasted according to the Specialty Coffee Association (SCA) protocol at five time intervals (8, 9, 10, 11, and 12 min) starting fromtwo initial roasting temperatures (210 and 215 °C). The roasted coffee was evaluated using thesensory analysis protocol of the SCA, which assessed fragrance/aroma; flavor, aftertaste, acidity, body, and balance; sweetness, uniformity, and cleanliness. In addition, we quantified the color of the coffee bean using a colorimeter. Sensory evaluation indicated that the best treatment was 215 °C/10min, with a predominant vanilla aroma (balsamic-sweet aromatic family) and an L* value of 13, suggesting a medium roast that may retain its functional properties. All treatments yielded values within proposed standards for a medium roast.Item IPR Pérola: Dwarf Arabica coffee cultivar with high resistance to leaf rust and large beans(Crop Breeding and Applied Biotechnology, 2024-10-04) Sera, Gustavo Hiroshi; Sera, Tumoru; Mariucci Junior, Valdir; Shigueoka, Luciana Harumi; Bortolato, Kawana SilvaIPR Pérola is a Coffea arabica cultivar developed from a cross between IAPAR 59 and Mundo Novo IAC 376-4. It features a dwarf-medium size, high yield, excellent cup quality, a medium-early ripening cycle, and large beans. Additionally, it boasts high resistance to coffee leaf rust.
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