Ciência Rural
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Item Steps of cryopreservation of coffee seeds: physiological responses and antioxidant systems(Universidade Federal de Santa Maria, 2023-08-21) Souza, Ana Cristina de; Costa, Marina Chagas; Figueiredo, Madeleine Alves de; Pereira, Cristiane Carvalho; Coelho, Stefânia Vilas Boas; Vilela, Ana Luiza Oliveira; Pereira, Diego de Souza; Rosa, Sttela Dellyzete Veiga Franco daThe cryopreservation of plant germplasm at ultralow temperatures is an alternative technique for the long-term storage of seeds of the genus Coffea sp. However, for this technique to be successful, cell integrity must be maintained at all stages of the process on the basis of scientific research. The present study investigated validated cryopreservation protocols for Coffea arabica L. seeds and evaluate the effects on the physiological and biochemical characteristics of the seeds at each stage of the process. Seeds were dried on silica gel or with saturated saline solution, precooled or not in a biofreezer, immersed in nitrogen, and reheated in a water bath. After each of these steps, the physiological and biochemical quality of the seeds was determined. Pre-cooling is a step that can be dispensed with in the cryopreservation of Coffea arabica seeds, direct immersion in liquid nitrogen being more indicated. Coffea arabica L. seeds tolerate cryopreservation after rapid drying in silica gel up to water contents of 17 or 20% (wb), with greater survival at 17%. The enzyme activities of catalase, polyphenol oxidase and peroxidase are indicators of the quality of C. arabica L. seeds subjected to cryopreservation.Item Chemical analyses of flowers and leaves for nutritional diagnoses of coffee trees(Universidade Federal de Santa Maria, 2021) Zabini, André Vinicius; Martinez, Herminia Emilia Prieto; Neves, Júlio César Lima; Cruz, Cosme Damião; Valadares, Samuel VasconcelosThe chemical analysis of flowers has been studied for some crops. In coffee trees, the flower tissue analysis could anticipate the nutritional diagnosis. This study aimed to: (i) compare the mineral composition of coffee flowers and leaves; and to (ii) generate reference values for nutritional diagnosis of coffee trees, based on flower and leaf analysis. Nutrient content of flowers and leaves and coffee productivity were evaluated in 26 commercial farms located in Manhuaçu, MG, Brazil throughout three years. The critical nutrient content range in Flowers are respectively: 2.78 – 3.17, 0.23 – 0.28, 2.80 – 3.12, 0.30 – 0.37, 0.24 – 0.30, 0.15 – 0.18 dag kg-1 of N, P, K, Ca, Mg, and S; and 17 – 21, 12 – 18, 52 – 80, 26 – 43, and 28 – 48 mg kg-1 of Zn, Cu, Mn, Fe, and B. For leaves, the critical nutrient ranges are respectively: 2.63 – 2.86, 0.13 – 0.14, 2.13 – 2.33, 1.04 – 1.22, 0.27 – 0.33, 0.15 – 0.18 dag kg-1 of N, P, K, Ca, Mg, and S; and 9 – 14, 15 – 23, 80 – 115, 99 – 148, and 31 – 37 mg kg-1 of Zn, Cu, Mn, Fe, and B. The nutritional diagnosis of coffee trees for N, P, Ca, Fe, Cu, and Mn can be anticipated using flower analysis.