Biblioteca do Café
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Item Selection for durable resistance to leaf rust using test-crosses on IAPAR-59 and Tupi IAC 1669-33 cultivars of Coffea arabica(Instituto de Tecnologia do Paraná - Tecpar, 2007-07) Sera, Gustavo Hiroshi; Sera, Tumoru; Ito, Dhalton Shiguer; Azevedo, José Alves de; Mata, João Siqueira da; Doi, Deisy Saori; Ribeiro Filho, ClaudionorThe aim of this study was to identify plants of the IAPAR-59 and Tupi IAC 1669-33 coffee cultivars with less defeated resistance genes by the rust races present at IAPAR (Londrina, Paraná State, Brazil) using test-crosses. Eighteen test-crosses derived from hybridizations between 'IAPAR-59' or 'Tupi IAC 1669-33' with susceptible coffee to the rust disease were evaluated. Six hybrids were used as susceptible standards originated from hybridizations between two susceptible coffee plants. Many parental plants of the 'IAPAR-59' and 'Tupi IAC 1669-33' presented more defeated resistance genes against rust races present at IAPAR than others of these cultivars or the genes were in heterozygous, because of segregant susceptible plants observed in some test-crosses. The test-crosses were very efficient to identify plants with less defeated resistance genes to the H. vastatrix. Coffee plants considered resistants would must be made test-crosses to verify which plants presented less and/or more defeated genes in homozygous.Item Coffee resistance to the main diseases: leaf rust and coffee berry disease(Sociedade Brasileira de Fisiologia Vegetal, 2006-06-14) Silva, Maria do Céu; Várzea, Victor; Guerra-Guimarães, Leonor; Azinheira, Helena Gil; Fernandez, Diana; Petitot, Anne-Sophie; Bertrand, Benoit; Lashermes, Philippe; Nicole, MichelConsiderable success has been obtained in the use of classical breeding to control economically important plant diseases, such as the coffee leaf rust and the coffee berry disease (CBD). There is a strong consensus that growing genetically resistant varieties is the most appropriate cost effective means of managing plant diseases and is one of the key components of crop improvement. It has also been recognized that a better knowledge of both, the pathogens and the plant defence mechanisms will allow the development of novel approaches to enhance the durability of resistance. After a brief description of concepts in the field of plant disease resistance, we attempt to give a view of the research progress on coffee leaf rust and CBD concerned with the pathogens infection and variability, coffee breeding for resistance and coffee resistance mechanisms.Item Selection for durable resistance to leaf rust using test-crosses on IAPAR-59 and Tupi IAC 1669-33 cultivars of Coffea arabica(Instituto de Tecnologia do Paraná - Tecpar, 2007-10-05) Sera, Gustavo Hiroshi; Sera, Tumoru; Ito, Dhalton Shiguer; Azevedo, José Alves de; Mata, João Siqueira da; Doi, Deisy Saori; Ribeiro Filho, ClaudionorThe aim of this study was to identify plants of the IAPAR-59 and Tupi IAC 1669-33 coffee cultivars with less defeated resistance genes by the rust races present at IAPAR (Londrina, Paraná State, Brazil) using test-crosses. Eighteen test-crosses derived from hybridizations between 'IAPAR-59' or 'Tupi IAC 1669-33' with susceptible coffee to the rust disease were evaluated. Six hybrids were used as susceptible standards originated from hybridizations between two susceptible coffee plants. Many parental plants of the 'IAPAR-59' and 'Tupi IAC 1669-33' presented more defeated resistance genes against rust races present at IAPAR than others of these cultivars or the genes were in heterozygous, because of segregant susceptible plants observed in some test-crosses. The test-crosses were very efficient to identify plants with less defeated resistance genes to the H. vastatrix. Coffee plants considered resistants would must be made test-crosses to verify which plants presented less and/or more defeated genes in homozygous.Item Efeito de fungicidas triazóis sobre o controle da cercosporiose em mudas de cafeeiro(Instituto Biológico, 2011) Patricio, F. R. A.; Braghini, M. T.Fungicidas do grupo dos triazóis e suas misturas com inseticidas são convencionalmente empregados para o controle da ferrugem e do bicho mineiro na cultura do cafeeiro. Neste estudo avaliou-se o efeito desses produtos sobre o controle da cercosporiose, causada por Cercospora coffeicola, outra importante doença do cafeeiro. Três experimentos foram realizados em casa de vegetação com mudas de cafeeiro da cultivar Mundo Novo IAC 501, sendo os produtos aplicados na parte aérea e no substrato. Os produtos aplicados na parte aérea foram: epoxiconazol, epoxiconazol + piraclostrobina, tetraconazol, ciproconazol, oxicloreto de cobre, azoxistrobina e carbendazim e no substrato: ciproconazol + tiametoxam, triadimenol + dissulfotom e triadimenol. Testemunhas sem tratamento químico também foram incluídas nos três experimentos. As mudas foram inoculadas com suspensões preparadas com cinco isolados de C. coffeicola (3-4 conídios/mL). A incidência e a severidade da doença foram avaliadas de 20 até 56 dias após as inoculações. Com exceção do oxicloreto de cobre, todos os demais tratamentos foram eficientes no controle da cercosporiose. Os triazóis, com exceção do tetraconazol, apresentaram sintomas reversíveis de fitotoxicidade. Os produtos: ciproconazol + tiametoxam, epoxiconazol + piraclostrobina e carbendazim tiveram os maiores efeitos residuais, e mantiveram o controle da doença por até 56 dias após sua aplica ção. Os triazóis utilizados para o controle da ferrugem também são eficientes para o controle da cercosporiose do cafeeiro.Item IPR Pérola: Dwarf Arabica coffee cultivar with high resistance to leaf rust and large beans(Crop Breeding and Applied Biotechnology, 2024-10-04) Sera, Gustavo Hiroshi; Sera, Tumoru; Mariucci Junior, Valdir; Shigueoka, Luciana Harumi; Bortolato, Kawana SilvaIPR Pérola is a Coffea arabica cultivar developed from a cross between IAPAR 59 and Mundo Novo IAC 376-4. It features a dwarf-medium size, high yield, excellent cup quality, a medium-early ripening cycle, and large beans. Additionally, it boasts high resistance to coffee leaf rust.Item New and efficient AS-PCR molecular marker for selection of coffee resistant to coffee leaf rust(Crop Breeding and Applied Biotechnology, 2024-10-15) Almeida, Dênia Pires de; Carneiro, Deisy Guimarães; Caixeta, Eveline Teixeira; Alves, Danúbia Rodrigues; Zambolim, LaércioCoffee leaf rust, caused by Hemileia vastatrix, is the most important disease affecting Coffea arabica. The use of molecular markers through marker-assisted selection (MAS) is an effective approach to develop cultivars with multiple and durable resistance, which is economically and environmentally beneficial for this disease control. In this work, a new molecular marker based on allele-specific polymerase chain reaction (AS-PCR) associated with the quantitative trait locus (QTL) of the linkage group 5 (LG5), which confers resistance to races I, II and pathotype 001 of H. vastatrix, was developed. The CaRHv10-AS marker was validated in a segregating genetic mapping population of 247 F2 genotypes and demonstrated selection efficiency greater than 97% for genotypes with resistance to three races. Thus, this work provides a fast, robust, and affordable molecular marker for use in MAS, facilitating the development of coffee plants with multiple resistance and ensuring sustainable coffee production.Item New vegetation index for monitoring coffee rust using sentinel-2 multispectral imagery(Universidade Federal de Lavras, 2023-12-29) Castro, Gabriel Dumbá Monteiro de; Vilela, Emerson Ferreira; Faria, Ana Luísa Ribeiro de; Silva, Rogério Antônio; Ferreira, Williams Pinto MarquesCoffee Rust (Hemileia vastatrix) is considered the primary coffee disease in the world. The pathogenic fungus can find favorable environmental conditions in different countries, constantly threatening coffee producers. The previous detection of the incidence of coffee rust in a region is crucial because it provides an overview of the disease’s progress aiding in coffee plantations management. The objective of this work was the development of a vegetation index for remote monitoring of coffee rust infestation. Using satellite images from the MSI/Sentinel-2 collection, the Machine Learning classifier algorithm - Random Forest, and the cloud processing platform - Google Earth Engine, the most sensitives bands in coffee rust detection were determined, namely B4 (Red), B7 (Red Edge 3) and B8A (Red Edge 4). Thus, the Triangular Vegetation Index method was used to create a new vegetative index for remote detection of coffee rust infestation on a regional scale, named Coffee Rust Detection Index (CRDI). A linear regression model was created to estimate rust infestation based on the performance of the new index. The model presented a coefficient of determination (R²) of 62.5%, and a root mean square error (RMSE) of 0.107. In addition, a comparison analysis of the new index with eight other vegetative indices commonly used in the literature was carried out. The CRDI obtained the best performance in coffee rust detection among the others. This study shows that the new index CRDI has the robustness and general capacity to be used in monitoring coffee rust infestation on a regional scale.Item Coffea arabica clones resistant to coffee leaf miner(Crop Breeding and Applied Biotechnology, 2016-03) Guerreiro Filho, Oliveiro; Mendonça, Alex Paulo; Nonato, Juliana Vieira Almeida; Andrade, Vinícius Teixeira; Fatobene, Bárbhara Joana dos Reis; Braghini, Masako Toma; Prela-Pantano, AngelicaBrazilian arabica coffee production is based on a set of highly productive cultivars sexually propagated. All of them are susceptible to coffee leaf miner, Leucoptera coffeella, and most of them are also susceptible to coffee leaf rust, Hemileia vastatrix. Aiming to reduce the selection process of cultivars with multiple resistances to both biotic constraints, the Coffea arabica clones 760, 1059, 1064 and 1215 were evaluated under field conditions. The mean yield of four C. arabica clones was not statistically different from the experimental controls; however, the yield of clone 1059 was 21% higher than the control cultivar Obatã IAC 1669-20. Leaf miner resistance level in all the evaluated clones remained the same as the mother plant’s. However, the same did not occur for resistance to H. vastatrix, due to the emergence of new pathogen races. In summary, it is potentially feasible the adoption of clone 1059 as a new clonal cultivar resistant to coffee leaf miner.Item Assisted selection using molecular markers linked to rust resistance SH3 gene in Coffea arabica(Crop Breeding and Applied Biotechnology, 2023-10-25) Silva, Angelita Garbossi; Ariyoshi, Caroline; Shigueoka, Luciana Harumi; Pereira, Luiz Filipe Protasio; Sera, Gustavo HiroshiThe aim of this work was to validate markers linked to the SH3 gene of coffee leaf rust (CLR) resistance and use them in assisted selection. Initially, we validated the markers in genotypes already known to carry SH3. Next, we performed phenotype and genotype evaluation for resistance to CLR in coffee plants growing under field conditions. We used Arabica coffee progenies derived from BA-10, which carries the SH3 gene due to introgression of C. liberica. Three SCAR markers (SP-M16-SH3, BA-48-21O-f, and BA-124-12K-f) and one SSR marker (Sat244) linked to SH3 gene were used to amplify the coffee plants’ DNA. Our assessments of markers validation in resistant genotypes, SP-M16-SH3 and BA-124-12K-f, were efficient to identify the SH3 gene. These two markers were used to evaluate the progenies derived from BA-10 and were significantly linked to the phenotype evaluations. The SP-M16-SH3 marker was more efficient, with the advantage of being codominant.Item Aceleração do melhoramento do cafeeiro via seleção genômica : agilidade e eficácia no lançamento de novas cultivares(Embrapa Café, 2022-08) Caixeta, Eveline Teixeira; Resende, Marcos Deon Vilela; Alkimim, Emilly Ruas; Sousa, Tiago Vieira; Oliveira, Antonio Carlos Baião de; Pereira, Antonio Alves; Alves, Rodrigo Silva