Biblioteca do Café
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Resultados da Pesquisa
Item Unlocking the Probiotic with Antioxidant-Rich Potential from Wine Coffee: In vitro Screening and Characterization(Editora UFLA, 2025-05-19) Jatmiko, Yoga Dwi; Suharjono, Suharjono; Ardyati, Tri; Mustafa, Irfan; Mustamin, Aryan; Puja, Lintang Ratu; Arifah, Siti Nur; Atho'illah, Mochammad FitriFood companies are becoming interested in lactic acid bacteria (LAB) due to their promising health benefits. LAB can be isolated from several bioresources; nothing is known about the probiotic potential of fermented coffee products like wine coffee. The study aimed to determine the probiotic potential of LAB isolated from wine coffee with antioxidant properties. Characterization of probiotics included antioxidant activity, acidity resistance, bile salt tolerance, antibacterial activity, antibiotic sensitivity, and haemolytic activity. The promising probiotics were identified using 16S rDNA sequences. The current results demonstrated that twenty-five isolates were found in wine coffee, and all showed antioxidant activities. Isolate E1 has the highest antioxidant activity (64.31%) among others. Isolates D4 and E2 were proposed as potential probiotics due to survival at low pH (pH 2) and bile salt (2%), inhibit Bacillus cereus ATCC 1178, Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 29213, and Salmonella Typhimurium TRD1, and do not display haemolytic activity. Isolates D4 and E2 demonstrated resistant to Kanamycin and Aztreonam. Based on molecular identification, isolates E2 and D4 were identified as Lactiplantibacillus plantarum, E1 was identified as Lacticaseibacillus paracasei. These LAB isolates are promising probiotic candidates and require further validation of their strain-specific potential.Item Changes in the Chemical Compound and Sensory Profiles of Liberica Fermentation (Coffea liberica) with Cellulolytic Bacteria Alcaligenes sp and Exiguobacterium indicum(Editora UFLA, 2024-11-14) Tarigan, Indra Lasmana; Adriliana, Zairaini; Putri, Alya Adiningtyas; Febrianto, Noor Ariefandie; Latief, Madyawati; SutrisnoJambi Province, particularly in Tanjung Jabung Barat Regency, Indonesia, is a crucial producer of Liberica coffee known as Liberika Tungkal Komposit (Libtukom). This variety of Liberica coffee (Coffea liberica) thrives in lowland regions, particularly peatlands. It grows best in slightly acidic soil with a pH between 6.0 and 6.5, which helps the plant absorb nutrients and resist pests more effectively. A significant post-harvest method to improve the quality and yield of Liberica coffee involves fermentation using cellulolytic bacteria, Alcaligenes sp. And Exiguobacterium indicum, found in civet digestive tracts. Fermentation enhances the coffe’s flavor, aroma, and quality, mimicking that of civet coffee. The primary objective of this study was to refine the sensory characteristics of Liberica coffee, improve its proximate composition, preserve its bioactive compounds, and boost its antioxidant properties. The fermentation was conducted over 24 hours and 48 hours periods, with roasting temperatures set at light (170 ºC), medium (190 ºC), and dark (210 ºC). The process began with the preparation of bacterial inoculum, followed by fermentation, roasting, and grinding. Sensory evaluation, proximate analysis, and chemical profiling (via FTIR, LC-MS, antioxidant activity, and total phenolic content) were then performed. The findings revealed that fermented Liberica coffee exhibited improved sensory attributes, achieving specialty-grade status at medium roasting temperatures. There was a decrease in moisture and ash content, an increase in fat content, and a reduction in carbohydrate and protein levels. The FTIR analysis showed the preservation of existing compounds, while LC-MS identified bioactive components based on their retention times and molecular weights. Additionally, the fermented coffee demonstrated increased antioxidant activity and total phenolic content compared to non-fermented samples. This study highlights the potential of fermentation technology to enhance the quality and health benefits of Liberica coffee significantly.Item Chemical and microbiological characteristics of kombucha made from robusta cascara and green tea(Universidade Federal de Lavras, 2024-06-17) Parwiyanti; Nurrocmah, Siti Iliyo; Malahayati, NuraDried coffee peel or cascara as coffee waste is a potential ingredient that still contains phytochemical substances such as polyphenol and clorogenic acid. Cascara can be used in making kombucha. Kombucha is a functional drink made of fermenting tea and sugar with using symbiotic culture of bacteria and yeast (SCOBY) as starter. The addition of cascara and combination with green tea could potentially improve nutritional values on kombucha. This study aimed to evaluate the chemical and microbiological characteristics of kombucha made from robusta cascara and green tea. This study used Completely Randomized Design with one treatment factor namely combination of robusta cascara and green tea (100%:0%; 75%:25%; 50%:50%; 25%:75%; 0%:100%). The treatment repeated three times. The parameters observed were total polyphenol content, total titrated acid, total dissolved solid, pH, and total lactic acid bacteria. The result showed that combination of robusta cascara and green tea had significant effects on all parameters. The increased of green tea proportion in treatments could increase content of total titrated acid from 0.0032% (kombucha with 100% robusta cascara) to 0.0048% (kombucha with 100% green tea) whereas total dissolved solid from 0.0667% Brix to 9.1333% Brix and pH values from 3.6000 to 3.4667 slightly decreased in ombucha.The higher polyphenol content in the raw material the higher total polyphenol obtained in kombucha reflected on 100% green tea kombucha with 0.2245 mg GAE/mL. Total lactic acid bacteria of kombucha obtained at 3.3760 log CFU/mL to 4.3917 log CFU/mL.Item Physical and sensorial quality of yellow caturra coffee after a carbonic maceration process(Universidade Federal de Lavras, 2023-12-15) Hernández-Alcántara, Gloria; Alarcón-Gutiérrez, Enrique; Ronzón-Soto, Samuel; García-Pérez, José AntonioHigh prices for differentiated coffees with unique flavor profiles have spurred research into processing methods to standardize coffee production and improve quality. Fermentation is a biotechnological tool that may enhance the sensory profile of coffee and boost its overall quality. Carbonic maceration, which uses constant pressure CO2, can be used as a technique to differentiate coffees. In this study, the physical and sensorial qualities of Coffea arabica (yellow caturra variety) beans, following carbonic maceration, were assessed. The experiment was carried out during the harvest 2021/22 at La Joya farm. Data were analyzed for both the physical quality of the green coffee beans and the cup quality (sensorial) based on the fermentation process and duration using ANOVA, Friedman and U Mann-Whitney tests, with a significance level of alpha = 0.05. All analyses were conducted using SPSS software version 25. The final score was influenced by both carbonic maceration and the duration of fermentation, with scores for zero-day compositions being lower than those for 5, 10 and 15 days. A significant difference in primary defects, based on the duration of fermentation, was observed. In conclusion, carbonic maceration improved both coffee qualities at day 5 compared to day zero (natural process). However, the physical quality is negatively affected after 10 and 15 days, while the sensory quality increases respectively.Item Specialty coffees fermented at different altitudes: Influence of yeast co-inoculation on chemical and sensory composition(Universidade Federal de Lavras, 2021-04-26) Bressani, Ana Paula Pereira; Schwan, Rosane Freitas; Simão, João Batista Pavesi; Batista, Nádia NaraSpecialty coffees are gaining more space in the Brazilian market, and the changes in production, processing, marketing, and consumer culture are noticeable. Unquestionably, the use of selected starter cultures in the fermentation process improves the coffee quality. However, the microorganisms' behavior can be different depending on the variety, processing method, and altitude. Thus, this paper aims to (i) use metabolomics, chemical, and sensory methodologies to evaluate the quality of fermented coffees of the cultivar Catuaí vermelho IAC-44 at different altitudes (600 and 1,200 m) located in the Caparaó region. (ii) analyze consumers’ knowledge and perspectives on coffees and investigate how information can influence consumers’ sensory experience. For this, the coffee cherries were harvested manually and separated into batches of 20 kg, representing seven treatments plus control at each altitude. The starter cultures - Saccharomyces cerevisiae CCMA 0543, Candida parapsilosis CCMA 0544, and Torulospora delbrueckii CCMA 0684 - were inoculated, isolated, and in co-inoculation. The control was performed without inoculation. Fermentation of whole fruits lasted 72 h in closed bioreactors. Then, the coffees were transferred to the suspended terraces until 11- 12% moisture. Samples were collected and frozen for chemical analysis. We studied the improving coffee beverage quality cultivated at low altitudes through inoculation of yeast during fermentation in dry processing. The analyzes were performed using liquid and gas chromatography, Fourier transforms infrared spectroscopy (FTIR), and cup testing. Differences in chemical compounds were observed between the inoculated coffees and the control, both for green and roasted beans. Fermentation improved the quality of low-altitude coffees, and the co-inoculation of non-Saccharomyces yeasts (C. parapsilosis CCMA 0544 and T. delbrueckii CCMA 0684) had the highest sensory score (85), being the most suitable for this process. The second topic addressed in this work was the influence of fermentation with starter cultures concerning the bioactive, chemical, and sensory compounds of coffee produced at high altitudes. The content of total polyphenols and antioxidants is strongly correlated with the end of fermentation in a closed bioreactor and after roasting. The trigonelline content has a moderate and negative correlation with fermentation and roasting. Fruity, citric, and wine notes were found only in inoculated treatments. The co-inoculated with the three yeasts showed the highest sensory score (86.9). The third aspect addressed was understanding the perspectives and desires of coffee consumers through a questionnaire in a digital format with 1,005 participants. The chocolate flavor is still the most expected in specialty coffee. The Check-all-that-Apply (CATA) test was carried out with 101 consumers using the same coffee (without and with information). Sensory analysis showed that consumers could be influenced by information. Therefore, co-inoculation of yeasts in coffee fermentation is a promising alternative for low and high-altitude coffee. Also, specialty fermented coffees should be made more widely available to consumers.Item Análise sensorial e química de cafés fermentados submetidos a diferentes processos de desinfecção do fruto(Universidade Federal de Lavras, 2023-04-14) Freitas, Renan Bertoli de; Pimenta, Carlos José; Carvalho, Gladyston RodriguesO Brasil é o maior produtor e exportador de café do mundo. Nos últimos anos, a população passou a valorizar cafés de qualidade superior, os chamados cafés especiais, e desde então os cafeicultores buscam melhorar o produto, desde o plantio até o processamento. A fermentação do café pode auxiliar no desenvolvimento de sabor e aroma, estabelecendo mais um caminho dentro dos processamentos pós colheita para melhorar a qualidade dos cafés brasileiros. Entretanto, a presença de microrganismos indesejáveis durante o processamento e fermentação do café pode influenciar negativamente na qualidade da bebida final. O objetivo com este trabalho foi avaliar química e sensorialmente amostras de café fermentado da cultivar MGS Paraíso 2 após desinfecção dos frutos por soluções sanitizantes. O delineamento utilizado foi DIC, com seis tratamentos e cinco repetições, totalizando 30 parcelas. Os tratamentos foram compostos pela testemunha (ausência de solução desinfetante – T1), T2 com utilização de ozônio como desinfetante, T3 com hipoclorito de sódio + ácido ascórbico, T4 com ácido peracético, T5 com metabissulfito de potássio e T6 com peróxido de hidrogênio. Após o período de fermentação, secagem, descanso e beneficiamento, as amostras foram encaminhadas para as análises sensoriais e químicas. De acordo com as análises sensoriais, todos os tratamentos apresentaram nota final acima de 84 pontos e a utilização das soluções desinfetantes não influenciou na qualidade da bebida em nenhum dos tratamentos. Dentre os sabores e aromas mais citados na análise CATA, adocicado, frutado e cítrico apresentaram destaque. Soluções como hipoclorito de sódio + ácido ascórbico e peróxido de hidrogênio podem diminuir o pH e, consequentemente, aumentar a acidez da bebida. A utilização de ozônio permitiu a preservação de sólidos solúveis totais, apresentou baixos níveis de condutividade elétrica e lixiviação de potássio. O peróxido de hidrogênio não constitui um sanitizante adequado para desinfecção de frutos de café, pois diminui o pH da solução e provoca maiores níveis de lixiviação de potássio. Já os sanitizantes ácido peracético e metabissulfito de potássio não apresentaram diferenças em relação a testemunha em nenhum dos aspectos físico-químicos analisados, mantendo as características da bebida. Desta forma, dentre as soluções sanitizantes, apenas o ozônio obteve poder eficiente de diminuir os microrganismos.Item Application of microrganisms for coffee fermentation(Universidade Federal de Lavras, 2018-03-23) Ribeiro, Luciana Silva; Schwan, Rosane Freitas; Evangelista, Suzana Reis; Miguel, Maria Gabriela da Cruz PedrozoArabica coffees of three varieties present distinct results when inoculated with microorganisms. The attempt to improve coffee quality can be implemented at different stages of coffee production. Presence of microorganisms during coffee fermentation directly interferes with the quality and characteristics of final beverage. The aim of this study was to evaluate the effect on coffee quality of inoculation of different microorganisms and also to study a bacteriological composition of coffees processed by wet fermentation. Three varieties of coffees studied at work: Ouro amarelo, Mundo novo and Catuaí Vermelho, mining region (Cerrado Mineiro and Southern of Minas Gerais). Ouro amarelo and Novo mundo processed by semi-dry method inoculated with three yeast strains (Saccharomyces cerevisiae CCMA 0200 and CCMA 0543; Torulaspora delbrueckii CCMA 0684). Two sensory analysis techniques were used (Cup Test and Temporal Dominance of Sensations). It was possible to observe that Ouro Amarelo variety obtained higher scores for evaluated attributes. The use of the CCMA 0543 and CCMA 0684 strains improved the sensory quality of the beverages. The second aspect discussed in this work is the diversity and effect of the inoculation of bacteria in fermentation of coffee by the wet process. Initially, a bacterial diversity was studied (Ouro amarelo, Mundo Novo and Catuaí Vermelho). Through identification techniques such as MALDI-TOF (Array Assisted Laser Desorption / Ionization - Time of Flight) and sequencing it was possible to identify 41 species of bacteria. In addition, some target compounds were quantified through HPLC (High Performance Liquid Chromatography) and CG-MS (Gas Chromatography and a mass spectrometer). According to the results, Lactobacillus plantarum and Leuconostoc mesenteroides were found in all varieties. Citric acid was detected at high concentrations and as the main classes of volatiles found were acids, alcohols, aldehydes and hydrocarbons. The sensorial profile demonstrates the presence of sensations of acidity (Ouro amarelo and Catuaí vermelho), bitterness, chocolate and nuts (Novo mundo) and sweetness (Catuaí vermelho). And finally, some species tested for their fermentation capacity to be applied as a starter culture in coffee of the Catuaí vermelho, in wet fermentation process. Strains Pantoea dispersa CCMA 1203; Cellulosimicrobium cellulans 1186, L. mesenteroides CCMA 1105 and L. plantarum CCMA 1065 presented better results in relation to acid production and changes in volatile profile during fermentation. These strains are strongly selective to be as well as starter cultures in wet fermented coffee. However, it is concluded that the inoculation of microorganisms in coffee is a highly promising alternative, being dependent on the variety and type of processing to be applied.Item Aspectos microbiológicos, perfil químico e sensorial de cafés fermentados em biorreatores(Universidade Federal de Lavras, 2020-03-04) Pereira, Thayanna Scopel; Schwan, Rosane Freitas; Batista, Nádia NaraO café é uma das commodities mais negociadas em todo o mundo e apresenta grande importância econômica no Brasil, principal produtor e exportador mundial. No setor cafeeiro, a pós-colheita do café é relevante por conferir impactos significativos na qualidade química e sensorial da bebida. Nesta etapa, o processo de fermentação ocorre de forma espontânea, através dos microrganismos presentes no fruto e no ambiente. No entanto, devido à falta de controle e padronização da qualidade, novos métodos para a condução da fermentação são necessários para a modulação do perfil sensorial da bebida final. O objetivo da pesquisa foi investigar a diversidade microbiana em diferentes fermentações utilizando a mesma metodologia, avaliar o perfil químico e sensorial de cafés fermentados em biorreatores, em diferentes regiões produtoras do estado de Minas Gerais, Brasil. Duas cidades no Sul de Minas (Carmo de Minas e Três Pontas), uma em Matas de Minas (Lajinha) e outra no Cerrado (Monte Carmelo). Dois processos foram realizados, café natural e café descascado (CD). A população de bactérias e leveduras foram avaliadas por plaqueamento em superfície. Os compostos químicos foram analisados por Ressonância Magnética Nuclear (RMN de 1H). A análise sensorial foi conduzida pelo método TDS (Temporal Dominance of Sensations). Houve diferença significativa na contagem populacional entre os diferentes tempos e entre os processamentos. A região Sul de Minas apresentou contagens superiores durante a fermentação. As leveduras Hanseniaspora opuntiae, Torulaspora delbrueckii, Pichia kluyveri, foram detectadas apenas nas cidades de Carmo de Minas (CM), Três Pontas (TP) e Lajinha (LAJ), respectivamente. Em Monte Carmelo (MC), Saccharomyces cerevisiae foi detectada em maior abundância. As bactérias Zymomonas mobilis e Leuconostoc lactis foram detectadas somente em CM. Acinetobacter johnsonii, A. oryzae e Enterobacter cloacae foram detectadas apenas em TP. Enterobacter hormaechei foi detectada apenas em LAJ. Bacillus cereus em MC. A composição química e o perfil sensorial foram influenciados pela alteração da forma de fermentação. A RMN de 1H, juntamente com a análise de componentes principais (PCA), mostraram agrupamento entre os cafés fermentados em biorreatores e o controle. As curvas de TDS variaram de acordo com a região e com o tipo de processamento pós-colheita realizado. Dentre as cidades estudadas, no café natural em MC e no CD em LAJ, os atributos caramelo e chocolate foram dominantes e específicas, respectivamente. Os atributos caramelo e amadeirado foram detectados apenas em MC, no café natural e café descascado, respectivamente. A dominância do atributo frutado foi comum em LAJ no café natural e em CM e TP no café descascado. A condução da fermentação em batelada, em ambiente fechado, com limitação de oxigênio e anaerobiose induzida pela microbiota epifítica, afetou a microbiota e alterou a composição química e sensorial. A microbiota apresentou comportamento diferente nas distintas variedades. A diversidade em cada fazenda indicou uma microbiota específica para a região geográfica e influenciou os sabores. Os cafés fermentados em biorreatores apresentaram mais compostos químicos precursores de sabores e aromas, e foram correlacionados ao café verde, e mais atributos sensoriais foram percebidos em relação ao controle.Item Avaliação sensorial e química de café natural com inoculação e controle de secagem(Universidade Federal de Lavras, 2022-07-28) Pereira, Vinicius Moribe; Borém, Flávio Meira; Andrade, Ednilton Tavares deO Brasil é o maior produtor de café arábica do mundo, e o mercado de cafés especiais também tem ganhado importância. Em média, esses cafés são comercializados com ágio de 27,1%. Para ser considerado especial. o café deve expressar um potencial elevado de aroma e sabor, além de uma qualidade intrínseca que é proveniente da interação entre genótipo, ambiente e processamento. Desta forma, o objetivo deste trabalho foi avaliar a qualidade de grãos de café natural com e sem inoculação de leveduras com tempos de fermentação diferentes e a cinética de secagem de dois métodos: secagem contínua e secagem intermitente. O método de secagem, após a fermentação, afeta a qualidade de bebida do café, visto que a qualidade do café fermentado e seco, via secagem intermitente, foi superior com o tempo efetivo de secagem inferior. A fermentação combinada com a secagem contínua intensifica atributos negativos da bebida do café. A fermentação mais prolongada contribuiu, para aumentar os conteúdos dos ácidos succínico e acético, na redução da concentração de açúcares e maior concentração de ácidos graxos linolênico, behemico, esteárico, araquidico e oleico. Para o café natural, nas secagens contínua e intermitente, os modelos matemáticos que melhor descrevem os dados experimentais de teor de água de equilíbrio foram os modelos de Dois Termos e Henderson & Pabis modificado.Item Improving the quality of kombucha cascara with different varieties and fermentation time in diverse arabica coffee (Coffea arabica L) cultivars(Universidade Federal de Lavras, 2023-01-12) Rohaya, Syarifah; Multahadi; Sulaiman, IsmailOne of the innovations of cascara is the kombucha cascara which is made from the fermented coffee fruit skin, which is fermented with a kombucha starter or Symbiotic Culture of Bacteria and Yeast (SCOBY). The cascara used in this study was cascara from the Timtim, Borbor, and Ateng super coffee varieties. This study aims to determine the effect of different varieties of arabica coffee and fermentation time on the quality of kombucha cascara. This study used a factorial randomized block design method consisting of two factors. The first factor is cascara from coffee varieties consisting of three levels (Timtim, Borbor, Ateng super). The second factor is the length of fermentation, consisting of three levels (4, 8, and 12 days). Parameters analyzed were antioxidant activity, total phenol, tannin content, pH, and total microbe. The results showed that cascara from coffee varieties significantly affected antioxidant activity, and pH. Fermentation time significantly affects antioxidant activity, total phenol, tannin content, pH, and total microbial. The best treatment was obtained in the cascara treatment of the Ateng super coffee variety and the fermentation time was 12 days with the following characteristics; antioxidant activity 45.74%, total phenol 132,59 mg/L, tannin content 0.46%, pH value 2.69, and total microbes 4.99x104 CFU/ml.