Biblioteca do Café

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    Induction and differentiation of reproductive buds in Coffea arabica L.
    (Brazilian Journal of Plant Physiology, 2005-06) Majerowicz, Nidia; Söndahl, Maro R.
    The induction and differentiation phases of reproductive buds of Coffea arabica L. have not received much attention. In the present paper, axillary buds from five plagiotropic branches that developed in the same growing season without fruits (1st crop branches), and from green segments of five recently grown plagiotropic branches with fruits (2nd crop branches), were collected every two weeks during successive inductive months of the year. This study was carried out with adult arabica trees, Catuaí Vermelho cv. IAC 81, cultivated under normal farming conditions in the region of Campinas, SP, Brazil (22º54' Lat. S). Slides of longitudinal-axial sections of 10-12 mum thickness were mounted for the characterization and quantification of histological stages of bud differentiation. The results indicate that the regulatory signals controlling the phases of induction and differentiation of floral buds are distinct, and that there are differences in the response between branches with and without fruits. In the case of 1st crop branches (no fruits present), induction of floral buds took place in January and February, whereas floral bud differentiation was observed during the months of March and April. In 2nd crop branches (fruits present), the induction of floral buds was observed during any month of the year provided that they had already overcome their juvenile state (October-July, in this study). In these 2nd crop branches, the flower bud differentiation was only observed after harvesting all pre-existing fruits of each branch (after May, in this study), which suggests that floral bud differentiation in Arabica coffee is influenced by the source-sink relationship, i.e. by the presence of developing fruits within each plagiotropic branch.
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    Ochratoxigenic fungi associated with green coffee beans (Coffea arabica L.) in conventional and organic cultivation in Brazil
    (Sociedade Brasileira de Microbiologia, 2013-10-09) Rezende, Elisângela de Fátima; Borges, Josiane Gonçalves; Cirillo, Marcelo Ângelo; Prado, Guilherme; Paiva, Leandro Carlos; Batista, Luís Roberto
    The genera Aspergillus comprises species that produce mycotoxins such as aflatoxins, ochratoxins and patulin. These are cosmopolitan species, natural contaminants of agricultural products. In coffee grains, the most important Aspergillus species in terms of the risk of presenting mycotoxins belong to the genera Aspergillus Section Circumdati and Section Nigri. The purpose of this study was to assess the occurrence of isolated ochratoxigenic fungi of coffee grains from organic and conventional cultivation from the South of Minas Gerais, Brazil, as well as to evaluate which farming system presents higher contamination risk by ochratoxin A (OTA) produced by fungi. Thirty samples of coffee grains (Coffea arabica L.) were analysed, being 20 of them of conventional coffee grains and 10 of them organic. The microbiological analysis was done with the Direct Plating Technique in a Dichloran Rose Bengal Chloramphenicol Agar (DRBC) media. The identification was done based on the macro and micro morphological characteristics and on the toxigenic potential with the Plug Agar technique. From the 30 samples analysed, 480 filamentous fungi of the genera Aspergillus of the Circumdati and Nigri Sections were isolated. The ochratoxigenic species identified were: Aspergillus auricoumus, A. ochraceus, A. ostianus, A. niger and A. niger Aggregate. The most frequent species which produces ochratoxin A among the isolated ones was A. ochraceus, corresponding to 89.55%. There was no significant difference regarding the presence of ochratoxigenic A. ochreceus between the conventional and organic cultivation systems, which suggests that the contamination risk is similar for both cultivation systems.
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    Predictive model of moisture content in dry parchment coffee beans using near-infrared spectroscopy (FT-NIR)
    (Editora UFLA, 2025-02-13) Lozano, Kevy Sandrith Ordoñez; Monje, Andrés Felipe Bahamón; Guzman, Nelson Gutierrez
    Moisture content is a key quality parameter in grain storage. Near infrared spectroscopy (NIRS) is a non-destructive technique, with fast and simple measurements, that can be a method to implement for monitoring this parameter. The objective of this research was to evaluate the NIRS technique for the prediction of the moisture content of dry samples of dry parchment coffee (DPC), for this it was necessary to carry out the study in different drying times in order to obtain several points of moisture of the grain that could exist, once the spectrum is taken in the FT-NIR, it is taken to the oven so that through the gravimetric method the real moisture of the grain can be known. The Spectrum Quant software was used to develop the predictive mathematical model by means of principal component regression (PCR) and partial least squares (PLS), using a set of randomly divided data for calibration and validation. The results showed that a better goodness of fit was found with a PLS model and the application of the baseline and second derivative correction, obtaining a coefficient of determination (R2) of 0.99 and a predictive standard error (SEP) of 0.34. Finding a good correlation between the real data with those estimated by the NIR equipment, emerging a fast and practical way in full-scale monitoring in DPC grain moisture control.
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    Bioactive Diterpenes and Serotonin Amides in Cold-Pressed Green Coffee Oil (Coffea arabica L.)
    (Sociedade Brasileira de Química, 2024-02-09) Silva, Rodrigo M. V.; Brand, Ana Laura M.; Tinoco, Natalia A. B.; Freitas, Suely P.; Rezende, Claudia M.
    Cold pressing is an environment-friendly mechanical extraction for oils from seeds. In this work, cold-pressed green Arabica coffee oil was investigated related to the influence of the pressing variables (preheating, exit diameter, screw speed, and particle size) on the chemical oil composition, mainly on the diterpenes and, for the first time in the scientific literature, on the content of serotonin amides (N-alkanoyl-5-hydroxytryptamides (Cn-5HT)). The oil yield from screw pressing varied from 2.65 to 6.27%, with major yields obtained as the size of the particle and temperature increased. Soxhlet extraction produced 9.46 ± 0.04% of oil. The fatty acid content of the oils varied from 32.79 to 33.49% and showed no significant difference among the different pressing conditions. The amount of the diterpenes kahweol and cafestol ranged from 13.33 to 16.72 mg g-1 and 37.11 to 47.14 mg g 1 of oil, respectively, summing 50.44 to 63.86 mg g 1 of diterpenes. The total content of Cn-5HTs ranged from 307.92 to 1716.52 µg g 1, being 114.42 to 577.37 µg g 1 for arachidic acid-5-hydroxytryptamide, (C20-5HT) and 193.50 to 1068.08 µg g 1 for behenic acid-5-hydroxytryptamide (C22-5HT) in oil, the most abundant in coffee bean. From the 16 cold press treatments, six conditions showed significant amounts of these compounds. Aspects related to the biological activity and relevance of coffee lipid diterpenes and Cn-5HTs are discussed.
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    Soil moisture space-time analysis to support improved crop management
    (Editora UFLA, 2015) Silva, Bruno Montoani; Santos, Walbert Junior Reis dos; Oliveira, Geraldo César de; Lima, José Maria de; Curi, Nilton; Marques, João José
    The knowledge of the water content in the soil profile is essential for an efficient management of crop growth and development. This work aimed to use geostatistical techniques in a spatio-temporal study of soil moisture in an Oxisol in order to provide that information for improved crop management. Data were collected in a coffee crop area at São Roque de Minas, in the upper São Francisco River basin, MG state, Brazil. The soil moisture was measured with a multi-sensor capacitance (MCP) probe at 10-, 20-, 30-, 40-, 60- and 100-cm depths between March and December, 2010. After adjusting the spherical semivariogram model using ordinary least squares, best model, the values were interpolated by kriging in order to have a continuous surface relating depth x time (CSDT) and the soil water availability to plant (SWAP). The results allowed additional insight on the dynamics of soil water and its availability to plant, and pointed to the effects of climate on the soil water content. These results also allowed identifying when and where there was greater water consumption by the plants, and the soil layers where water was available and potentially explored by the plant root system.
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    Productivity and physical quality of grains from Coffea arabica L. in a tropical high-altitude climate in Brazil
    (Universidade Federal de Lavras, 2023-11-19) Martins, Adriana Novais; Turco, Patricia Helena Nogueira; Araújo, Humberto Sampaio de; Firetti, Ricardo
    The coffee plant is extremely important for Brazilian agribusiness as it generates foreign exchange earnings and employment throughout the production chain. In this study, we evaluated the production and parameters related to the quality of 18 Coffea arabica L. cultivars grafted onto the Apoatã IAC 2258 cultivar, in seven consecutive harvests. The experiment was installed in 2014 and carried out for seven harvests, in Fazenda Recreio in the municipality of Vera Cruz, state of São Paulo, Brazil. We used a randomised block design (DBC), with three experimental replications, which were conducted in 54 plots. We analysed productivity, yield, and grain size parameters. The data were evaluated by the analysis of variance (ANOVA) and the mean values were used to conduct the Scott-Knott and Tukey test. The results showed that in all the cycles, the characteristics evaluated showed significant differences among the cultivars. The ratio between the residual mean squares of the analysis of variance was less than 7:1 for all the characteristics evaluated, which allowed us to compare the seven harvests with each other. The cultivars IPR 100, Obatã IAC 1669–20, IPR 107 and IAC 125 RN performed the best, as determined by the parameters evaluated, and thus, we recommend these cultivars for growing coffee tropical high-altitude climate in Brazil.
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    Post-harvest of coffee: factors that influence the final quality of the beverage
    (Revista Engenharia na Agricultura, 2022-04-27) Silva, Camilla Sena da; Coelho, Ana Paula de Freitas; Lisboa, Cristiane Fernandes; Vieira, Gerival; Teles, Maria Carolina de Abreu
    Brazil is the world’s largest producer and exporter of coffee and the second largest consumer market. Brazil offer differentiated and high quality products to maintain its leading position in coffee production and export. However, Brazilian coffee has suffered some limitations in its commercialization in the international market, due to qualitative aspects. The development of taste and aroma is highly complex, since hundreds of chemical reactions take place at the same time, such as the breakdown of proteins, polysaccharides, trigonelline and chlorogenic acid, mainly in the roasting process. Then, it is necessary that, in addition to pre-harvest management, there are post-harvest practices that meet consumer demands regarding the final quality of the beverage. Studies on physical and chemical changes in the composition of coffee beans must continue to be performed in a comprehensive manner, since factors such as damage to coffee beans, drying methods, processing, storage time, type of packaging and chemical components are directly related to sensorial properties and thus define the quality of the beverage at this point of view. Thus, the objective of this review was to relate the physicochemical, biochemical and physiological characteristics of coffee beans after harvest with the quality of the product after roasting.
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    Mudas propagadas vegetativamente de Coffea arabica tratadas com extratos de algas
    (Universidade Federal de Lavras, 2022-07-28) Azevedo, Harianna Paula Alves de; Santos, Heloisa Oliveira dos; Carvalho, Samuel Pereira; Melo, Paulo César
    A cafeicultura é de suma importância para todo o mundo. No Brasil, destacou-se econômica e socialmente desde a chegada das primeiras mudas e sementes. Com boa e rápida adaptação ao solo e clima, o café brasileiro tomou grandes proporções na balança comercial internacional. Porém, com as mudanças nos hábitos do consumidor, se tem buscado alternativas para os cultivos mais sustentáveis. Uma opção para esses cultivos é o uso de bioestimulantes que são de origem natural, como os extratos de algas. Assim, o objetivo foi avaliar o desenvolvimento de mudas de café arábica propagadas assexuadamente, quando tratadas com extratos de algas. O trabalho foi composto por dois experimentos independentes, conduzidos no Setor de Cafeicultura do Departamento de Agricultura, utilizando de mudas pré-formadas propagadas assexuadamente, da cultivar Mundo Novo. O primeiro foi estabelecido em delineamento em blocos casualizados, em esquema fatorial 4 x 3, com quatro dosagens (0; 2,5; 5 e 10 mL.L-¹) de extratos das algas Ascophyllum nodosum, Lithothamnium ssp. e Kappaphicus spp. O segundo experimento foi estabelecido em blocos casualizados, testando a mistura desses extratos na dose de 5 mL.L-¹. Em ambos foram avaliadas a altura do broto, área foliar, matéria seca foliar, matéria seca do caule, número de folhas, níveis relativos de clorofilas a, b e total, condutância estomática foliar, densidade e funcionalidade estomática. Para as avaliações radiculares determinou-se a massa seca da raiz, comprimento, volume e área superficial radicular, e o diâmetro da raiz. Para a dose de 5 mL.L-¹ houve melhores resultados para os três extratos de algas, Ascophyllum nodosum e Lithothamnium ssp. com os melhores resultados para as principais características de parte aérea. Houve resultados satisfatórios para Ascophyllum nodosum e Lithothamnium ssp. no enraizamento na dose de 5 a 10 mL.L-¹. Para Kappaphicus spp. o efeito foi negativo. As combinações dos extratos de algas não exercem efeito em mudas de café arábica.
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    Polymorphic information content of SSR markers for Coffea spp.
    (Crop Breeding and Applied Biotechnology, 2010) Caixeta, Eveline Teixera; Missio, Robson Fernando; Zambolim, Eunize Maciel; Zambolim, Laércio; Cruz, Cosme Damião; Sakiyama, Ney Sussumu
    Thirty-three coffee SSR primers from enriched genomic library with (GT)15 and (AGG)10 repeats were analyzed in 24 coffee tree accessions. Twenty-two primers were polymorphic among accessions; the number of alleles ranged from 2 to 13, with the mean number of 5.1 alleles per primer. PIC values ranged from 0.08 to 0.79. The highest mean PIC values were found for C. canephora (0.46), and the lowest values for C. arabica (0.22) and triploids (0.22) accessions. The polymorphic SSR markers used in this study were useful for genetic fingerprinting in the coffee tree, especially in the C. canephora and the leaf rust resistant arabica cultivars.
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    Potential of Metarhizium from soils and rhizosphere of diversified coffee systems in insect suppression and its indirect effect on a coffee pest through endophytic association
    (Universidade Federal de Viçosa, 2021-10-18) Franzin, Mayara Loss; Venzon, Madelaine; Elliot, Simon Luke; Fadini, Marcos Antonio Matiello; Moreira, Camila Costa
    Diversified crop systems enhance ecosystem services, such as pest biological control, via attraction and maintenance of entomophagous, and improve soil chemical and biological characteristics. Soil entomopathogenic fungi, such as Metarhizium spp., are also benefit in diversified systems, because these systems reduce direct soil irradiation and increase soil humid. The fungi of this genera are important microbial control agents of insect pests, which made them an important tool to pest management. Besides, this genus is also considered as endophytic, colonizing roots of plants, increasing plant growth and promoting protection against pests. Here, we designed a strategic diversified coffee system in the Cerrado of Minas Gerais, by the associating coffee with plants that attract natural enemies, and investigated the effects of such diversification in the Metarhizium conservation in soil. Coffee was associated with trees of Inga edulis (Fabaceae) and Senna macranthera (Fabaceae) and perennial bushes of Varronia curassavica (Cordiaceae). Non-crop plants were maintained between coffee rows, except before the coffee harvesting, as they can provide food resources for predators and parasitoids. As control, we designed plots with conventional coffee monoculture, with the standard conventional use of pesticides. In Chapter 1, we evaluated Metarhizium activity and density, soil chemical characteristics and enzymatic activity from diversified and conventional soil of coffee systems over time. We estimated the coffee yield in both coffee systems by measuring the volume of coffee fruits per plant and the weight of 100 fruits. The activity and density of Metarhizium spp. was assessed using the bait survival technique with Tenebrio molitor (Coleoptera: Tenebrionidae) larvae as bait, and plating soil suspensions on to solid selective media for counts of colony-forming units (CFU), respectively. Twelve months after experiment starts, bait insects died two times faster due to Metarhizium spp. in the diversified than in the conventional coffee systems, while the density of Metarhizium spp. was also higher in the diversified. Beta-glycosidase activity was greater in the diversified coffee system, indicating more organic matter decomposition and carbon cycle in this coffee system. Furthermore, there was no difference of volume of fruit between coffee systems, however, the weight of 100 fruits was higher in diversified system in 2021. In Chapter 2, we investigatedMetarhizium spp. rhizosphere association with plant roots from diversified and conventional coffee system. We used the same protocols of Chapter 1 to evaluated Metarhizium activity and density. Both were higher in soil and roots samples from diversified than conventional system. We isolated Metarhizium spp. from I. edulis, V. Curassavica, S. macranthera, Gnaphalium spicatum, Conyza bonariensis, Solanum Americanum, Ageratum conyzoides, Bidens pilosa, Sida cordifolia and coffee plants of diversified coffee system. In Chapter 3, we investigate whether Metarhizium robertsii and Metarhizium brunneum, isolated from coffee roots from diversified coffee system in Cerrado, improve coffee seedlings growth and indirectly protect them against the coffee leaf miner (CLM) Leucoptera coffeella (Lepidoptera: Lyonetiidae). We recovered Metarhizium spp. from most of coffee seedling roots, indicating that these fungi can colonize coffee roots by soil drench. We found that M. robertsii increased the leaf area of coffee seedlings. Besides, the plants inoculated with Metarhizium isolates had lower percentage of mined leaf area and the development time of CLM was higher in these plants. Therefore, in this thesis we showed that strategic plant diversification in coffee crops increases density and activity of Metarhizium, soil enzymatic activity and coffee yield over time. Besides, M. brunneum and M. robertsii have potential to use as inoculates of coffee seedlings, because the plants inoculated with them isolates showed lower CLM damage. Therefore, strategic plant diversification in coffee systems can be an alternative to monocultures, improving soil quality and coffee yield.