Brazilian Archives of Biology and Technology

URI permanente para esta coleçãohttps://sbicafe.ufv.br/handle/123456789/12090

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Caffeine Formation by Suspension Cultures of Coffea dewevrei
(Instituto de Tecnologia do Paraná - Tecpar, 2000-01) Sartor, Rosana Mary; Mazzafera, Paulo
The low caffeine content in leaves of C. dewevrei (~ 0.5 mg/g) is due to a low biosynthesis associated with a fast degradation. On the other hand, high biosynthesis and low degradation confer a higher content (~ 8 mg/g) in leaves of C. arabica. In this work it was observed that cell cultures of C. dewevrei recovered the ability to synthesize caffeine almost in similar levels of C. arabica cultures. Tracer experiments with labelled carbon dioxide showed a significant accumulation of radioactivity in caffeine and metabolites, indicating an active biosynthesis. When the cultures were fed with labelled caffeine most of the radioactivity was recovered in caffeine, indicating that although active, degradation was not so efficient as in leaves, and therefore, contributing for the alkaloid accumulation in the cell cultures.
Influence of coffee pruning on the severity of frost damage
(Instituto de Tecnologia do Paraná - Tecpar, 2000-03) Androcioli Filho, Armando; Caramori, Paulo Henrique
Frost damages in a field experiment of pruning types and systems for the cultivars of Coffea arabica Catuaí and Mundo Novo, were evaluated at Londrina (23 o 22’S, 52 o 10 ́W), State of Parana, southern Brazil, during the winter of 1990 and 1994. Pruning types evaluated were ‘esqueletamento’ (cutting off all plagiotropic branches at 20-30 cm from the orthotropic branch), ‘decote’ (cutting off the orthotropic branch at 1.5 m and 2.0 m above ground) and ‘recepa’ (cutting off the orthotropic branch at 0.8 m above ground), performed on all rows and on alternate rows, and on different sections of the plant. Results indicated that frost damage could increase according to the type and height of pruning. The pruning type ‘esqueletamento’ and prunings at higher levels were more suitable for regions with frost risk. Under severe frost condition, pruning type did not affect the damage in anyone of the treatments evaluated.
Compositional changes of proteins and amino acids in germinating coffee seeds
(Instituto de Tecnologia do Paraná - Tecpar, 2000-07) Shimizu, Milton Massao; Mazzafera, Paulo
Endosperm is the main reserve tissue in coffee seeds. Coffee (Coffea arabica L.) seeds were germinated for six weeks and qualitative and quantitative changes in amino acids and proteins were investigated. The total content of free amino acids were reduced during germination, however, protein content remained constant. SDS-PAGE profiles showed that legumin-like proteins became less stained in the last weeks. Asparagine, glutamic acid, aspartic acid, alanine and lysine were the major free amino acids, although serine and glutamine were also significant. Except for tyrosine, which increased with germination, all other amino acids were reduced. Analysis of the amino acid composition of the total soluble protein showed glutamic acid/glutamine and glycine as the main amino acids. However, other amino acids such as leucine, aspartic acid/asparagine, alanine, lysine, serine were also found in reasonable amounts.
Production of Flammulina velutipes on coffee husk and coffee spent-ground
(Instituto de Tecnologia do Paraná - Tecpar, 2001-06) Leifa, Fan; Pandey, Ashok; Soccol, Carlos R.
Solid state cultivation (SSC) was carried out to evaluate the feasibility of using coffee husk and spent-ground as substrates for the production of edible mushroom Flammulina under different conditions of moisture and spawn rate. The strain of F. velutipes LPB 01 was adapted for a coffee husk extract medium. Best results were obtained with 25% spawn rate, though there was not much difference when lower spawn rates (10-20%) were used. Ideal moisture content for mycelial growth was 60% and 55% for coffee husk and spent-ground, respectively. With coffee husk as substrate, first fructification occurred after 25 days of inoculation and the biological efficiency reached about 56% with two flushes after 40 days. With spent-ground as substrate, first fructification occurred 21 days after inoculation and the biological efficiency reached about 78% in 40 days. There was decrease in the caffeine and tannins contents (10.2 and 20.4%, respectively) in coffee husk after 40 days. In coffee spent-ground, the tannin contents decreased by 28% after 40 days. These decrease was attributed to the degradation of caffeine or tannins by the culture because these were not adsorbed in the fungal mycelia. Results showed the feasibility of using coffee husk and coffee spent-ground as substrate without any nutritional supplementation for cultivation of edible fungus in SSC. Spent ground appeared better than coffee husk.
Evaluation of arsenic and selenium in brazilian soluble coffee by inductively coupled plasma atomic emission spectrometry with hydride generation
(Instituto de Tecnologia do Paraná - Tecpar, 2001-10) Santos, Éder José dos; Oliveira, Elisabeth de
A method for the evaluation of arsenic and selenium in soluble coffee by inductively coupled plasma atomic emission spectrometry with continuous hydride generation to attend the Brazilian food legislation is described. Samples were digested with nitric acid and hydrogen peroxide in a focused microwave system. Slow heating eliminated nitric acid and selenium (VI) was reduced to selenium (IV) by addition of 6 mol/L hydrochloric acid and heating at 90°C under a reflux system. The influence of sample acidity on sensitivity was investigated. Hydrochloric acid 6 mol/L was the most suitable reaction medium. Practical detection limits of 2.0μg/L for As and 1.0μg/L for Se were achieved and attended the Brazilian food legislation. The results of recoveries on spiked samples demonstrate the reliability and accuracy of the procedure.
Effect of leaf water potential on cold tolerance of Coffea arabica L.
(Instituto de Tecnologia do Paraná - Tecpar, 2002-12) Caramori, Lázara Pereira Campos; Caramori, Paulo Henrique; Manetti Filho, João
Young coffee plants from cultivar Mundo Novo of Coffea arabica were grown without irrigation for 32 consecutive days, to evaluate the effect of leaf water potential on damage caused by low temperatures, under controlled conditions. À wide range of leaf water potentials were evaluated, from — 0.45MPa (wet soil) at the beginning of the experimental period, to — 4.8MPa (severe leaf wilting) at the end. Results showed that under moderate water stress, there was a higher frequency of undamaged plants and lower frequency of severely damaged plants. These results help explain part of the regional variability observed after a frost and stress the importance of new studies associating cold and drought tolerance in coffee.
Dietary utilisation of protein and energy from fresh and ensiled coffee pulp by the nile tilapia, oreochromis niloticus
(Instituto de Tecnologia do Paraná - Tecpar, 2003-03) Moreau, Yann; Arredondo, Jose-Luis; Gaime, Isabelle Perraud; Roussos, Sevastianos
Dietary protein and energy utilisation of diets containing fresh and ensiled coffee pulp were studied on 3.2 ± 0.2 g Nile tilapia for 28 days. Diets formulation and feeding were designed on the basis of daily dietary protein and energy allowance. A control diet A (100 % protein and 100 % energy allowance) corresponding to 15 g CP kg -1 day -1 and 750 kJ kg -1 day -1 , a low protein control diet B (80 % protein and 100 % energy allowance), two diets C and E (100 % protein and 100 % energy allowance) where 20 % of protein were supplied by coffee pulp, and two diets D and F with the same amount of coffee pulp than in C and E and supplementation in non-protein energy. Inclusion of coffee pulp in the diet strongly impaired growth and feed utilisation. Silage process improved overall feed utilisation comparing to fresh coffee pulp. Results showed that fresh or ensiled coffee pulp was not a suitable feedstuff for Nile tilapia. However, better knowledge on modification occurring during silage process could allow finding the way to significantly improve nutritive value of coffee pulp by-products.
Varietal identification of coffee seeds by RAPD technique
(Instituto de Tecnologia do Paraná - Tecpar, 2004-03) Crochemore, Maria Lúcia; Nunes, Liliane Moreira; Andrade, Giselly Aparecida; Molinari, Hugo Bruno Correa; Vasconcellos, Maria Elizabeth
This study aimed the identification of cultivars and/or lines of Coffea arabica of commercial interest, using PCR-RAPD markers. The DNA of ground seeds lots of 12 cultivars and/or lines were evaluated with five primers (Operon OPA 01, OPA 04, OPG 11, OPY 16, and OPX 09) were obtained from a selection of 56 primers. The electrophoretic profiles allowed distinction among eight cultivars and/or lines as well as heterogeneity between and within lots of IAPAR59.
High metal contents in coffee plant organs developed in tubets with different proportions of biosolid composts and carbonized rice hulls
(Instituto de Tecnologia do Paraná - Tecpar, 2004-08) Franco, Elias; Miglioranza, Edison; Fonseca, Ésio de Pádua
The objective of this study was to analyze the effect of biosolid composts (BC) and carbonized rice hull (RH) on the production of Coffea arabica L. cv Catuaí Vermelho seedlings (IAC H 2077-2-5-99) grown in tubets. A totally randomized experimental design with five treatments and four replications was conducted in a nursery , to evaluate Co, Ni, Cr, Pb, Cu, Mn and Zn contents in roots, stems and leaves. Higher contents of Cr, Co, Ni, Pb, Cu and Zn were found in roots, while leaves carried the highest content of Mn. Zn in leaves went from average to high in plants developed in BC. In plants developed in RH. Mn content was about four times higher than the adequate concentration. Ni, Co, Cr, Pb and Cu contents found in leaves were considered normal, according to international standards. The best treatment for coffee seedling nutrition was 50% RH + 50% BC.
Modifications on leaf anatomy of Coffea arabica caused by shade of pigeonpea (Cajanus cajan)
(Instituto de Tecnologia do Paraná - Tecpar, 2004-11) Morais, Heverly; Medri, Moacyr Eurípedes; Marur, Celso Jamil; Caramori, Paulo Henrique; Ribeiro, Ana Maria de Arruda; Gomes, José Carlos
Modifications on leaf anatomy in Coffea arabica shaded with pigeonpea (Cajanus cajan), compared to cultivation under full sun, were assessed. The leaves fully exposed to sunlight presented thicker cuticles and cellular walls, narrower epidermis cells, palisade parenchyma with longer cells, thicker lacunar parenchyma, fewer intercellular spaces and a larger stomata number. Leaves under dense shade presented a narrower cuticle and cellular wall; a mesophyll with smaller volume, but with larger intercellular spaces; and epidermis with thicker cells and a smaller stomata amount, surrounded by subsidiary cells of smaller dimensions. Plants grown under full sunlight presented higher values of net photosynthesis. The results evidenced that the species C. arabica has a wide range of phenotypic adaptation to changes in the radiation intensity.
Genetic variability for frost resistance among coffea accessions assessed in the field and in a cold chamber
(Instituto de Tecnologia do Paraná - Tecpar, 2005-01) Petek, Marcos Rafael; Sera, Tumoru; Alteia, Marcos Zorzenon
The genetic variability for frost resistance was evaluated in field conditions and cold chamber among Coffea accessions. Results showed that C. liberica var. dewevrei and C. racemosa, as well as hybrids obtained from these species were more resistant to frost. There was a great genetic variability for frost resistance among the evaluated sources (b 2 = 0,98). The correlation estimated between damages in the field and in the cold chamber was 0.933**, therefore, this methodology was efficient and feasible and could be potentially used in a breeding program.
Genetic diversity among forty coffee varieties assessed by rapd markers associated with restriction digestion
(Instituto de Tecnologia do Paraná - Tecpar, 2005-07) Diniz, Leandro Eugênio Cardamoni; Ruas, Claudete de Fátima; Carvalho, Valdemar de Paula; Torres, Fabrício Medeiros; Ruas, Eduardo Augusto; Santos, Melissa de Oliveira; Sera, Tumoru; Ruas, Paulo Maurício
The genetic variability of 40 accessions of C. arabica was evaluated using a combination of the random amplified polymorphic DNA (RAPD) technique and restriction digestion of genomic DNA. The genetic variability and the relatedness among all accessions were initially evaluated using 195 RAPD primers which revealed a very low level of genetic variation. To improve the efficiency in the detection of polymorphism, the genomic DNA of all accessions were submitted to digestion with restriction endonucleases prior to PCR amplification. A total of 24 primers combined with restriction digestion of DNA rendered 318 bands, of which 266 (83.65%) were polymorphic. The associations among genotypes were estimated using UPGMA-clustering analysis. The accessions were properly clustered according to pedigree and agronomic features. The ability to distinguish among coffee accessions was greater for RAPD plus restriction digestion than for RAPD alone, providing evidences that the combination of the techniques was very efficient for the estimation of genetic relationship among C. arabica genotypes.
Production of herbicide-resistant coffee plants (Coffea canephora P.) via Agrobacterium tumefaciens-mediated transformation
(Instituto de Tecnologia do Paraná - Tecpar, 2006-01) Ribas, Alessandra Ferreira; Kobayashi, Adilson Kenji; Pereira, Luiz Filipe Protasio; Vieira, Luiz Gonzaga Esteves
Transgenic plants of Coffea canephora P. resistant to the herbicide ammonium glufosinate were regenerated from leaf explants after co-culture with Agrobacterium tumefaciens strain EHA105 harboring pCambia3301, a plasmid that contains the bar and the uidA genes both under control of 35S promoter. Direct somatic embryogenesis was induced on basal medium contained 1⁄4 strength macro salts and half strength micro salts of MS medium, organic constituents of B 5 medium and 30 g.L -1 sucrose supplemented with 5 μ M N 6 – (2-isopentenyl)-adenine (2-iP). Ten μ M ammonium glufosinate was used for putative transgenic somatic embryos selection. Presence and integration of the bar gene were confirmed by PCR and Southern blot analysis. Selected transgenic coffee plants sprayed with up to 1600 mg.L -1 of Finale  , a herbicide containing glufosinate as the active ingredient, retained their pigmentation and continued to grow normally during ex vitro acclimation.
A comparison between enzyme immunoassay and HPLC for ochratoxin A detection in green, roasted and instant coffee
(Instituto de Tecnologia do Paraná - Tecpar, 2007-03) Fujii, Simone; Ono, Elisabete Yurie Sataque; Ribeiro, Ricardo Marcelo Reche; Assunção, Fernanda Garcia Algarte; Takabayashi, Cássia Reika; Oliveira, Tereza Cristina Rocha Moreira de; Itano, Eiko Nakagawa; Ueno, Yoshio; Kawamura, Osamu; Hirooka, Elisa Yoko
An indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) for ochratoxin A (OTA) detection in green, roasted and instant coffees was developed using anti-OTA monoclonal antibody. Immunological reagents prepared were OTA-BSA (4.76 μ g/mL), anti-OTA.7 MAb (2x10 3 -fold dilution) and HRP-anti IgG (10 3 -fold dilution). The detection limit was 3.73 ng OTA/g and correlation coefficients (r) between this immunoassay and high performance liquid chromatography were 0.98 for green coffee, 0.98 for roasted and 0.86 for instant. OTA levels detected by ic- ELISA were higher than by HPLC, with ELISA/HPLC ratio of 0.66 - 1.46 (green coffee), 0.96 - 1.11 (roasted) and 0.93 - 1.82 (instant). ELISA recoveries for OTA added to coffee (5 - 70 ng/g) were 81.53 % for green coffee, 46.73 % for roasted and 64.35 % for instant, while recoveries by HPLC were 80.54 %, 45.91 % and 55.15 %, respectively. Matrices interferences were minimized by samples dilution before carrying out the ELISA assay. The results indicate that MAb-based ic-ELISA could be a simple, sensitive and specific screening tool for OTA detection, contributing to quality and safety of coffee products.
Production of extracellular protease by a brazilian strain of Beauveria bassiana reactivated on coffee berry borer, Hypothenemus hampei
(Instituto de Tecnologia do Paraná - Tecpar, 2007-03) Ito, Eliana Tiemi; Pereira, Geni Varéa-; Miyagui, Dalva Tomoe; Pinotti, Maria Helena Pimenta; Neves, Pedro Manoel Oliveira Janeiro
Studies were carried out on extracellular protease production by Beauveria bassiana CG432 in liquid medium containing glucose and yeast extract. B. Bassiana presented active growth after lag period of 24 h., produced 80% of the total of the extracellular protease activity in 48 h which was maximum on the 5 th culture day. The extracellular protease presented optimum activity at 60 o C, was stable up to 1M Cl - , maintained the stability during 15 day at 4 o C and -18 o C, but was not stable if frozen repeatedly.
Partial resistance to fruit necrosis associated to Colletotrichum spp. among arabic coffee genotypes
(Instituto de Tecnologia do Paraná - Tecpar, 2007-05) Sera, Gustavo Hiroshi; Sera, Tumoru; Ito, Dhalton Shiguer; Azevedo, José Alves de; Ribeiro Filho, Claudionor; Mata, João Siqueira da
The aim of this study was to evaluate the resistance to fruit necrosis associated to Colletotrichum spp. in Coffea arabica L.. Twenty-two arabic coffee genotypes were performed in a randomized block design, with three replications and ten plants per plot. They were evaluated for the variables fruit necrosis, luminosity index inside of the plant canopy and fruits per productive nodes of the plants. There was genetic variability for the resistance to fruit necrosis. Different partial resistance levels, varying from highly susceptible (score = 3.89) to moderately resistant (score = 1.77) were observed. The genotypes that presented moderate resistance were the cultivars IPR- 100, IPR-103, IPR-105 and IPR-108 and the coffee selection IAPAR-00023.
Partial resistance to fruit necrosis associated to Colletotrichum spp. among arabic coffee genotypes
(Instituto de Tecnologia do Paraná - Tecpar, 2007-05) Sera, Gustavo Hiroshi; Sera, Tumoru; Ito, Dhalton Shiguer; Azevedo, José Alves de; Ribeiro Filho, Claudionor; Mata, João Siqueira da
The aim of this study was to evaluate the resistance to fruit necrosis associated to Colletotrichum spp. in Coffea arabica L.. Twenty-two arabic coffee genotypes were performed in a randomized block design, with three replications and ten plants per plot. They were evaluated for the variables fruit necrosis, luminosity index inside of the plant canopy and fruits per productive nodes of the plants. There was genetic variability for the resistance to fruit necrosis. Different partial resistance levels, varying from highly susceptible (score = 3.89) to moderately resistant (score = 1.77) were observed. The genotypes that presented moderate resistance were the cultivars IPR-100, IPR-103, IPR-105 and IPR-108 and the coffee selection IAPAR-00023.
Selection for durable resistance to leaf rust using test-crosses on IAPAR-59 and Tupi IAC 1669-33 cultivars of Coffea arabica
(Instituto de Tecnologia do Paraná - Tecpar, 2007-07) Sera, Gustavo Hiroshi; Sera, Tumoru; Ito, Dhalton Shiguer; Azevedo, José Alves de; Mata, João Siqueira da; Doi, Deisy Saori; Ribeiro Filho, Claudionor
The aim of this study was to identify plants of the IAPAR-59 and Tupi IAC 1669-33 coffee cultivars with less defeated resistance genes by the rust races present at IAPAR (Londrina, Paraná State, Brazil) using test-crosses. Eighteen test-crosses derived from hybridizations between ‘IAPAR-59’ or ‘Tupi IAC 1669-33’ with susceptible coffee to the rust disease were evaluated. Six hybrids were used as susceptible standards originated from hybridizations between two susceptible coffee plants. Many parental plants of the ‘IAPAR-59’ and ‘Tupi IAC 1669- 33’ presented more defeated resistance genes against rust races present at IAPAR than others of these cultivars or the genes were in heterozygous, because of segregant susceptible plants observed in some test-crosses. The test- crosses were very efficient to identify plants with less defeated resistance genes to the H. vastatrix. Coffee plants considered resistants would must be made test-crosses to verify which plants presented less and/or more defeated genes in homozygous.
Selection for durable resistance to leaf rust using test-crosses on IAPAR-59 and Tupi IAC 1669-33 cultivars of Coffea arabica
(Instituto de Tecnologia do Paraná - Tecpar, 2007-07) Sera, Gustavo Hiroshi; Sera, Tumoru; Ito, Dhalton Shiguer; Azevedo, José Alves de; Mata, João Siqueira da; Doi, Deisy Saori; Ribeiro Filho, Claudionor
The aim of this study was to identify plants of the IAPAR-59 and Tupi IAC 1669-33 coffee cultivars with less defeated resistance genes by the rust races present at IAPAR (Londrina, Paraná State, Brazil) using test-crosses. Eighteen test-crosses derived from hybridizations between 'IAPAR-59' or 'Tupi IAC 1669-33' with susceptible coffee to the rust disease were evaluated. Six hybrids were used as susceptible standards originated from hybridizations between two susceptible coffee plants. Many parental plants of the 'IAPAR-59' and 'Tupi IAC 1669-33' presented more defeated resistance genes against rust races present at IAPAR than others of these cultivars or the genes were in heterozygous, because of segregant susceptible plants observed in some test-crosses. The test-crosses were very efficient to identify plants with less defeated resistance genes to the H. vastatrix. Coffee plants considered resistants would must be made test-crosses to verify which plants presented less and/or more defeated genes in homozygous.
Resistance to leaf rust in coffee carrying SH3 gene and others SH genes
(Instituto de Tecnologia do Paraná - Tecpar, 2007-09) Sera, Gustavo Hiroshi; Sera, Tumoru; Ito, Dhalton Shiguer; Azevedo, José Alves de; Mata, João Siqueira da; Dói, Deisy Saori; Ribeiro Filho, Claudionor; Kanayama, Fabio Seidi
The aim of this work was to evaluate the resistance to rust in coffee carrying SH3 gene and other SH genes. Twenty one CIFC’s coffee trees with several resistance genes SH were evaluated in field conditions. All the evaluated coffees carrying SH3 gene presented resistance to the rust. It was possible that rust races with the virulence gene v3 in the Paraná State didn’t exist. The SH3 gene in combination with genes SH5, SH6, SH7, SH8, SH9 and SH? would be very important to obtain cultivars with more durable resistance to the rust.