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Item Coffee seed physiology(Brazilian Journal of Plant Physiology, 2006-06) Eira, Mirian T. S.; Silva, E. A. Amaral da; Castro, Renato D. De; Dussert, Stéphane; Walters, Christina; Bewley, J. Derek; Hilhorst, Henk W. M.Considerable advances in our understanding of coffee seed physiology have been made in recent years. However, despite intense research efforts, there are many aspects that remain unclear. This paper gives an overview of the current understanding of the more important features concerning coffee seed physiology, and provides information on recent findings on seed development, germination, storage and longevity.Item Cytology, biochemistry and molecular changes during coffee fruit development(Brazilian Journal of Plant Physiology, 2006-06-14) Castro, Renato D. De; Marraccini, PierreIn commercial coffee species (Coffea arabica and Coffea canephora), fruit development is a lengthy process, characterized by tissue changes and evolutions. For example, soon after fecundation and up to mid development, the fruit is mainly constituted of the pericarp and perisperm tissue. Thereafter, the perisperm gradually disappears and is progressively replaced by the endosperm (true seed). Initially present in a "liquid" state, the endosperm hardens as it ripens during the maturation phase, as a result of accumulation of storage proteins, sucrose and complex polysaccharides representing the main reserves of the seed. The last step of maturation is characterized by the dehydration of the endosperm and the color change of the pericarp. Important quantitative and qualitative changes accompany fruit growth, highlighting the importance of its study to better understand the final characteristics of coffee beans. Following a description of the coffee fruit tissues, this review presents some data concerning biochemical, enzymatic and gene expression variations observed during the coffee fruit development. The latter will also be analyzed in the light of recent data (electronic expression profiles) arising from the Brazilian Coffee Genome Project.