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Reproducibility of the RAPD marker and its efficiency in coffee tree genotype grouping analysis

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dc.contributor.author Sakiyama, Ney Sussumu
dc.contributor.author Teixeira-Cabral, Terezinha Aparecida
dc.contributor.author Zambolim, Laércio
dc.contributor.author Pereira, Antonio Alves
dc.contributor.author Barros, Everaldo Gonçalves
dc.contributor.author Sakiyama, Cássia Camargo Harger
dc.date.accessioned 2024-05-27T22:37:17Z
dc.date.available 2024-05-27T22:37:17Z
dc.date.issued 2002
dc.identifier.citation SAKIYAMA, N. S. et al. Reproducibility of the RAPD marker and its efficiency in coffee tree genotype grouping analysis. Crop Breeding and Applied Biotechnology, Viçosa, v. 2, n. 1, p. 121-129, 2002. pt_BR
dc.identifier.uri https://cbab.sbmp.org.br/wp-content/uploads/2023/08/16-121.pdf pt_BR
dc.identifier.uri http://www.sbicafe.ufv.br/handle/123456789/14376
dc.description.abstract The genetic diversity of Coffea arabic L. cultivars is relatively narrow and its assessment and increase is important for breeding. Fifty two arbitrary primers were used to evaluate the reproducibility and the influence of the number of RAPD (Random Amplified Polymorphic DNA) markers on the estimation of genetic distances among 40 genotypes of Coffea spp. The average number of polymorphic bands was 6.69 per primer among all genotypes, and 1.27 among arabica coffee genotypes. RAPD markers were efficient in estimating the genetic distances among the genotypes. The increase in RAPD loci number during grouping analysis did not affect the major groups’ composition; however, it affected the composition of subgroups. Marker reproducibility was 76.88% and replicated data was recommended for distinguishing genotypes with the same genetic background. pt_BR
dc.format pdf pt_BR
dc.language.iso en pt_BR
dc.publisher Crop Breeding and Applied Biotechnology pt_BR
dc.rights Open Access pt_BR
dc.subject Coffea pt_BR
dc.subject DNA markers pt_BR
dc.subject Diversity pt_BR
dc.subject Coffee breeding pt_BR
dc.subject.classification Cafeicultura::Genética e melhoramento pt_BR
dc.title Reproducibility of the RAPD marker and its efficiency in coffee tree genotype grouping analysis pt_BR
dc.type Artigo pt_BR

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