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Gene expression during the germination of coffee seed

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dc.contributor.author Silva, Edvaldo Aparecido Amaral da
dc.contributor.author Acencio, Marcio Luis
dc.contributor.author Bovolenta, Luiz Augusto
dc.contributor.author Lemke, Ney
dc.contributor.author Varani, Alessandro de Mello
dc.contributor.author Bravo, Juliana Pereira
dc.contributor.author Hoshino-Bezerra, Andrea Akemi
dc.contributor.author Lemos, Eliana Gertrudes Macedo
dc.date.accessioned 2022-02-08T13:37:20Z
dc.date.available 2022-02-08T13:37:20Z
dc.date.issued 2019
dc.identifier.citation SILVA, E. A. A. et al. Gene expression during the germination of coffee seed. Journal of Seed Science, Londrina, v. 41, n. 2, p. 168-179, abr./jul. 2019. pt_BR
dc.identifier.issn 2317-1545
dc.identifier.uri http://dx.doi.org/10.1590/2317-1545v41n2208263 pt_BR
dc.identifier.uri http://www.sbicafe.ufv.br/handle/123456789/13289
dc.description.abstract Germination of the coffee (Coffea arabica L.) seed is the result of events that occur simultaneously in the embryo and endosperm. To understand the molecular mechanisms responsible for these events, we undertook a transcriptome analysis of embryo, micropylar and lateral endosperms from 10-day-imbibed seeds. The sequencing yielded contigs coding for 16,813 proteins. From those, 14,005 (~ 83%) were highly similar to at least one protein sequence in the nr database. 162 genes were significantly expressed in the embryo, 36 in the micropylar endosperm and 72 in the lateral endosperm. The tissue specificity analysis of the significantly expressed genes showed that the embryo had the highest proportion of specific genes (113/162, ~70%), while 11 were expressed in the micropylar and lateral endosperms. In the embryo, genes were mainly associated with abiotic stress, cell growth, and intercellular communication. In the micropylar and lateral endosperms, they were associated with abiotic stress and cell wall degradation. The accuracy of RNA-seq data was confirmed by RT-qPCR. This work adds new information about the molecular mechanism involved in coffee seed germination. pt_BR
dc.format pdf pt_BR
dc.language.iso en pt_BR
dc.publisher Associação Brasileira de Tecnologia de Sementes - ABRATES pt_BR
dc.relation.ispartofseries Journal of Seed Science;v.41, n.2, 2019
dc.rights Open Access pt_BR
dc.subject RNA-seq pt_BR
dc.subject Embrião pt_BR
dc.subject Endosperma micropilar pt_BR
dc.subject Endosperma lateral pt_BR
dc.subject Coffea arabica pt_BR
dc.subject.classification Cafeicultura::Sementes e mudas pt_BR
dc.title Gene expression during the germination of coffee seed pt_BR
dc.type Artigo pt_BR

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