Almeida, Dênia Pires deCarneiro, Deisy GuimarãesCaixeta, Eveline TeixeiraAlves, Danúbia RodriguesZambolim, Laércio2025-06-042024-10-15ALMEIDA, D. P. et al. New and efficient AS-PCR molecular marker for selection of coffee resistant to coffee leaf rust. Crop Breeding and Applied Biotechnology, Viçosa, v. 24, n. 04, p. 01-09, 15 oct. 2024.1984-7033http://dx.doi.org/10.1590/1984-70332024v24n4a47${dspace.url}/handle/123456789/14710Coffee leaf rust, caused by Hemileia vastatrix, is the most important disease affecting Coffea arabica. The use of molecular markers through marker-assisted selection (MAS) is an effective approach to develop cultivars with multiple and durable resistance, which is economically and environmentally beneficial for this disease control. In this work, a new molecular marker based on allele-specific polymerase chain reaction (AS-PCR) associated with the quantitative trait locus (QTL) of the linkage group 5 (LG5), which confers resistance to races I, II and pathotype 001 of H. vastatrix, was developed. The CaRHv10-AS marker was validated in a segregating genetic mapping population of 247 F2 genotypes and demonstrated selection efficiency greater than 97% for genotypes with resistance to three races. Thus, this work provides a fast, robust, and affordable molecular marker for use in MAS, facilitating the development of coffee plants with multiple resistance and ensuring sustainable coffee production.pdfenOpen AccessCoffee breedingQTLHemileia vastatrixSustainable coffeeMarker-assisted selection (MAS)Cafeicultura::Genética e melhoramentoArtigo