Santos-Briones, César De LosHernández-Sotomayor, S. M. Teresa2025-12-082006-06-14SANTOS-BRIONES, C.; HERNÁNDEZ-SOTOMAYOR, S. M. T. Coffee biotechnology. Brazilian Journal of Plant Physiology, Campos dos Goytacazes, v. 18, n. 1, p. 217-227, 2006.1677-9452https://doi.org/10.1590/S1677-04202006000100015sbicafe.ufv.br/handle-server/123456789/14854In the last three decades, interest has turned to in vitro cell culture in different areas of coffee research. In vitro techniques have been applied not only for coffee improvement through genetic transformation but also to study various aspects in coffee cells such as chemical (caffeine synthesis and the production of coffee aroma), physiological and more recently, biochemical aspects. The most important advances obtained to date on in vitro coffee techniques in fields like biochemistry, physiology, regeneration systems and genetic engineering, are presented and discussed.enCoffeaCoffeeGenetic transformationIn vitro tissue cultureRegenerationSomatic embryogenesisArtigo